Platelet gene expression as a biomarker risk stratification tool in acute myocardial infarction: A pilot investigation

David C. Calverley, Ivan P. Casserly, Qamrul G. Choudhury, Tzu L. Phang, Bifeng Gao, John C. Messenger, Mark W. Geraci

    Research output: Contribution to journalArticle

    2 Scopus citations

    Abstract

    Platelets play a major role in the pathophysiology of acute myocardial infarction (AMI). Recent evidence reveals megakaryocyte- derived platelet pre-mRNA is spliced to mRNA and then translated into functional proteins in response to external stimulation. An exon microarray analyzes pre-mRNA alternative splicing and is thus applicable for studying gene expression in the anucleate platelet. We hypothesized a subset of megakaryocyte/platelet genes exists that are significantly over or underexpressed in AMI compared with stable coronary artery disease (CAD), yielding a gene expression profile for further study. Microarray analysis employing platelet mRNA was used to generate gene expression data in the above two patient groups. Unsupervised hierarchical clustering has revealed an expression profile that includes 95 over- or under-expressed genes depicted in a heat map where separation of both sets takes place. This preliminary study reveals a platelet-based gene expression signature that differentiates between AMI and stable CAD, and further study may yield a prognostic tool for a future AMI event in atherosclerosis risk factor-based subsets of CAD patients.

    Original languageEnglish (US)
    Pages (from-to)9-15
    Number of pages7
    JournalClinical Medicine Insights: Blood Disorders
    Volume3
    StatePublished - Jan 1 2010

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    Keywords

    • Gene expression
    • Myocardial infarction
    • Platelets
    • Thrombosis

    ASJC Scopus subject areas

    • Hematology

    Cite this

    Calverley, D. C., Casserly, I. P., Choudhury, Q. G., Phang, T. L., Gao, B., Messenger, J. C., & Geraci, M. W. (2010). Platelet gene expression as a biomarker risk stratification tool in acute myocardial infarction: A pilot investigation. Clinical Medicine Insights: Blood Disorders, 3, 9-15.