Platelet factor 4 binds to vascular proteoglycans and controls both growth factor activities and platelet activation

Megan S. Lord, Bill Cheng, Brooke L. Farrugia, Simon McCarthy, John M. Whitelock

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Platelet factor 4 (PF4) is produced by platelets with roles in both inflammation and wound healing. PF4 is stored in platelet α-granules bound to the glycosaminoglycan (GAG) chains of serglycin. This study revealed that platelet serglycin is decorated with chondroitin/dermatan sulfate and that PF4 binds to these GAG chains. Additionally, PF4 had a higher affinity for endothelial-derived perlecan heparan sulfate chains than serglycin GAG chains. The binding of PF4 to perlecan was found to inhibit both FGF2 signaling and platelet activation. This study revealed additional insight into the ways in which PF4 interacts with components of the vasculature to modulate cellular events.

Original languageEnglish (US)
Pages (from-to)4054-4063
Number of pages10
JournalJournal of Biological Chemistry
Volume292
Issue number10
DOIs
StatePublished - Mar 10 2017
Externally publishedYes

Fingerprint

Platelet Factor 4
Platelet Activation
Proteoglycans
Platelets
Blood Vessels
Intercellular Signaling Peptides and Proteins
Chemical activation
Glycosaminoglycans
Blood Platelets
Chondroitin
Dermatan Sulfate
Heparitin Sulfate
Chondroitin Sulfates
Fibroblast Growth Factor 2
Wound Healing
Inflammation
serglycin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Platelet factor 4 binds to vascular proteoglycans and controls both growth factor activities and platelet activation. / Lord, Megan S.; Cheng, Bill; Farrugia, Brooke L.; McCarthy, Simon; Whitelock, John M.

In: Journal of Biological Chemistry, Vol. 292, No. 10, 10.03.2017, p. 4054-4063.

Research output: Contribution to journalArticle

Lord, Megan S. ; Cheng, Bill ; Farrugia, Brooke L. ; McCarthy, Simon ; Whitelock, John M. / Platelet factor 4 binds to vascular proteoglycans and controls both growth factor activities and platelet activation. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 10. pp. 4054-4063.
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