Platelet-derived growth factor ligands and receptors immunolocalized in proliferative retinal diseases

S. G. Robbins, R. N. Mixon, David Wilson, C. E. Hart, Joseph Robertson, I. Westra, Stephen Planck, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Purpose. Platelet-derived growth factor (PDGF) and its receptors could contribute to the development of proliferative retinal membranes, because PDGF is angiogenic and is both mitogenic and chemotactic for retinal pigment epithelial (RPE) and glial cells, components of membranes. The authors sought to determine whether PDGF ligands and their receptors were present in proliferative retinal membranes. Methods. To localize PDGF ligands and receptors; the authors examined normal postmortem control retinas, intact eyes with proliferative vitreoretinopathy (PVR) or proliferative diabetic retinopathy (PDR), and membranes removed by vitrectomy from patients with PVR, epimacular proliferation, PDR, or PVR with PDR of previous onset. Sections were stained with antibodies specific for each PDGF ligand and receptor, using an avidin-biotin-complex immunohistochemical technique. To correlate PDGF receptor β (PDGFRβ) and ligand immunostaining, sections were double labelled with antibodies specific for either PDGF-A or PDGF-B. Results. Ligands. In the normal retina and choroid, staining for the A-chain was limited to vascular cells. Only the nerve fiber layer and vessels were positive for the B-chain. In diseased tissue, PDGF-A immunoreactivity was detected as intense staining (+++) of all but one of the proliferative retinal membranes; some RPE cells were positive for PDGF-A, especially in the eye with PDR. PDGF-B was also present in many proliferative retinal membranes but not in RPE cells. Receptors. In the normal retina and choroid, both PDGFRα and PDGFRβ were detected only in vessels. In proliferative retinal membranes, both receptors were detected in vessels. Long strands of RPE-like cells at the edges of PVR membranes were strongly positive for PDGFRβ but negative or ±, respectively, for PDGFRα. Double-label assays showed that PDGFRβ was often colocalized with each PDGF ligand, especially in pigmented cells. Conclusions. PDGF ligands and receptors are widespread in proliferative retinal membranes of different origin. Because PDGFRβ and PDGF-B were colocalized in many of the same cells, the potential for autocrine and paracrine stimulation of cell migration and growth exists. These results are consistent with a role for PDGF ligands and receptors in the pathogenesis of different proliferative retinopathies.

Original languageEnglish (US)
Pages (from-to)3649-3663
Number of pages15
JournalInvestigative Ophthalmology and Visual Science
Volume35
Issue number10
StatePublished - 1994

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Platelet-Derived Growth Factor Receptors
Retinal Diseases
Ligands
Proliferative Vitreoretinopathy
Retinal Pigments
Membranes
Diabetic Retinopathy
Proto-Oncogene Proteins c-sis
Platelet-Derived Growth Factor
Epithelial Cells
Retina
Choroid
Staining and Labeling
Avidin
Antibodies
Vitrectomy
Cellular Structures
Biotin
Nerve Fibers
Neuroglia

Keywords

  • immunolocalization
  • PDGF
  • PDGF receptor
  • proliferative retinal membranes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Platelet-derived growth factor ligands and receptors immunolocalized in proliferative retinal diseases. / Robbins, S. G.; Mixon, R. N.; Wilson, David; Hart, C. E.; Robertson, Joseph; Westra, I.; Planck, Stephen; Rosenbaum, James (Jim).

In: Investigative Ophthalmology and Visual Science, Vol. 35, No. 10, 1994, p. 3649-3663.

Research output: Contribution to journalArticle

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abstract = "Purpose. Platelet-derived growth factor (PDGF) and its receptors could contribute to the development of proliferative retinal membranes, because PDGF is angiogenic and is both mitogenic and chemotactic for retinal pigment epithelial (RPE) and glial cells, components of membranes. The authors sought to determine whether PDGF ligands and their receptors were present in proliferative retinal membranes. Methods. To localize PDGF ligands and receptors; the authors examined normal postmortem control retinas, intact eyes with proliferative vitreoretinopathy (PVR) or proliferative diabetic retinopathy (PDR), and membranes removed by vitrectomy from patients with PVR, epimacular proliferation, PDR, or PVR with PDR of previous onset. Sections were stained with antibodies specific for each PDGF ligand and receptor, using an avidin-biotin-complex immunohistochemical technique. To correlate PDGF receptor β (PDGFRβ) and ligand immunostaining, sections were double labelled with antibodies specific for either PDGF-A or PDGF-B. Results. Ligands. In the normal retina and choroid, staining for the A-chain was limited to vascular cells. Only the nerve fiber layer and vessels were positive for the B-chain. In diseased tissue, PDGF-A immunoreactivity was detected as intense staining (+++) of all but one of the proliferative retinal membranes; some RPE cells were positive for PDGF-A, especially in the eye with PDR. PDGF-B was also present in many proliferative retinal membranes but not in RPE cells. Receptors. In the normal retina and choroid, both PDGFRα and PDGFRβ were detected only in vessels. In proliferative retinal membranes, both receptors were detected in vessels. Long strands of RPE-like cells at the edges of PVR membranes were strongly positive for PDGFRβ but negative or ±, respectively, for PDGFRα. Double-label assays showed that PDGFRβ was often colocalized with each PDGF ligand, especially in pigmented cells. Conclusions. PDGF ligands and receptors are widespread in proliferative retinal membranes of different origin. Because PDGFRβ and PDGF-B were colocalized in many of the same cells, the potential for autocrine and paracrine stimulation of cell migration and growth exists. These results are consistent with a role for PDGF ligands and receptors in the pathogenesis of different proliferative retinopathies.",
keywords = "immunolocalization, PDGF, PDGF receptor, proliferative retinal membranes",
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T1 - Platelet-derived growth factor ligands and receptors immunolocalized in proliferative retinal diseases

AU - Robbins, S. G.

AU - Mixon, R. N.

AU - Wilson, David

AU - Hart, C. E.

AU - Robertson, Joseph

AU - Westra, I.

AU - Planck, Stephen

AU - Rosenbaum, James (Jim)

PY - 1994

Y1 - 1994

N2 - Purpose. Platelet-derived growth factor (PDGF) and its receptors could contribute to the development of proliferative retinal membranes, because PDGF is angiogenic and is both mitogenic and chemotactic for retinal pigment epithelial (RPE) and glial cells, components of membranes. The authors sought to determine whether PDGF ligands and their receptors were present in proliferative retinal membranes. Methods. To localize PDGF ligands and receptors; the authors examined normal postmortem control retinas, intact eyes with proliferative vitreoretinopathy (PVR) or proliferative diabetic retinopathy (PDR), and membranes removed by vitrectomy from patients with PVR, epimacular proliferation, PDR, or PVR with PDR of previous onset. Sections were stained with antibodies specific for each PDGF ligand and receptor, using an avidin-biotin-complex immunohistochemical technique. To correlate PDGF receptor β (PDGFRβ) and ligand immunostaining, sections were double labelled with antibodies specific for either PDGF-A or PDGF-B. Results. Ligands. In the normal retina and choroid, staining for the A-chain was limited to vascular cells. Only the nerve fiber layer and vessels were positive for the B-chain. In diseased tissue, PDGF-A immunoreactivity was detected as intense staining (+++) of all but one of the proliferative retinal membranes; some RPE cells were positive for PDGF-A, especially in the eye with PDR. PDGF-B was also present in many proliferative retinal membranes but not in RPE cells. Receptors. In the normal retina and choroid, both PDGFRα and PDGFRβ were detected only in vessels. In proliferative retinal membranes, both receptors were detected in vessels. Long strands of RPE-like cells at the edges of PVR membranes were strongly positive for PDGFRβ but negative or ±, respectively, for PDGFRα. Double-label assays showed that PDGFRβ was often colocalized with each PDGF ligand, especially in pigmented cells. Conclusions. PDGF ligands and receptors are widespread in proliferative retinal membranes of different origin. Because PDGFRβ and PDGF-B were colocalized in many of the same cells, the potential for autocrine and paracrine stimulation of cell migration and growth exists. These results are consistent with a role for PDGF ligands and receptors in the pathogenesis of different proliferative retinopathies.

AB - Purpose. Platelet-derived growth factor (PDGF) and its receptors could contribute to the development of proliferative retinal membranes, because PDGF is angiogenic and is both mitogenic and chemotactic for retinal pigment epithelial (RPE) and glial cells, components of membranes. The authors sought to determine whether PDGF ligands and their receptors were present in proliferative retinal membranes. Methods. To localize PDGF ligands and receptors; the authors examined normal postmortem control retinas, intact eyes with proliferative vitreoretinopathy (PVR) or proliferative diabetic retinopathy (PDR), and membranes removed by vitrectomy from patients with PVR, epimacular proliferation, PDR, or PVR with PDR of previous onset. Sections were stained with antibodies specific for each PDGF ligand and receptor, using an avidin-biotin-complex immunohistochemical technique. To correlate PDGF receptor β (PDGFRβ) and ligand immunostaining, sections were double labelled with antibodies specific for either PDGF-A or PDGF-B. Results. Ligands. In the normal retina and choroid, staining for the A-chain was limited to vascular cells. Only the nerve fiber layer and vessels were positive for the B-chain. In diseased tissue, PDGF-A immunoreactivity was detected as intense staining (+++) of all but one of the proliferative retinal membranes; some RPE cells were positive for PDGF-A, especially in the eye with PDR. PDGF-B was also present in many proliferative retinal membranes but not in RPE cells. Receptors. In the normal retina and choroid, both PDGFRα and PDGFRβ were detected only in vessels. In proliferative retinal membranes, both receptors were detected in vessels. Long strands of RPE-like cells at the edges of PVR membranes were strongly positive for PDGFRβ but negative or ±, respectively, for PDGFRα. Double-label assays showed that PDGFRβ was often colocalized with each PDGF ligand, especially in pigmented cells. Conclusions. PDGF ligands and receptors are widespread in proliferative retinal membranes of different origin. Because PDGFRβ and PDGF-B were colocalized in many of the same cells, the potential for autocrine and paracrine stimulation of cell migration and growth exists. These results are consistent with a role for PDGF ligands and receptors in the pathogenesis of different proliferative retinopathies.

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