Abstract
Stimulation by platelet-derived growth factor (PDGF) is known to increase the number of IGF-I binding sites in cells in culture. We show here that PDGF also increases the levels of IGF-1 receptor mRNA. Using cell lines stably transfected with an expression plasmid in which the reporter luciferase gene is under the control of the rat IGF-1 receptor gene promoter, we find that PDGF increases the activity of this promoter. A short IGF-1 receptor gene promoter, comprising about 100 base pairs of the sequence immediately upstream of the initiation of transcription site, is sufficient for a response to the stimulatory action of PDGF. These results suggest that an increase in RNA levels and in promoter activity may play an important role in the increase in IGF-1 receptor levels that occurs after stimulation by PDGF.
Original language | English (US) |
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Pages (from-to) | 374-379 |
Number of pages | 6 |
Journal | Experimental Cell Research |
Volume | 211 |
Issue number | 2 |
DOIs | |
State | Published - Apr 1994 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology