Platelet-derived growth factor increases the activity of the promoter of the insulin-like growth factor-1 (IGF-1) receptor gene

Michele Rubini, Haim Werner, Enrico Gandini, Charles Roberts, Derek Leroith, Renato Baserga

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Stimulation by platelet-derived growth factor (PDGF) is known to increase the number of IGF-I binding sites in cells in culture. We show here that PDGF also increases the levels of IGF-1 receptor mRNA. Using cell lines stably transfected with an expression plasmid in which the reporter luciferase gene is under the control of the rat IGF-1 receptor gene promoter, we find that PDGF increases the activity of this promoter. A short IGF-1 receptor gene promoter, comprising about 100 base pairs of the sequence immediately upstream of the initiation of transcription site, is sufficient for a response to the stimulatory action of PDGF. These results suggest that an increase in RNA levels and in promoter activity may play an important role in the increase in IGF-1 receptor levels that occurs after stimulation by PDGF.

Original languageEnglish (US)
Pages (from-to)374-379
Number of pages6
JournalExperimental Cell Research
Volume211
Issue number2
DOIs
StatePublished - 1994
Externally publishedYes

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Somatomedin Receptors
Platelet-Derived Growth Factor
Genes
Transcription Initiation Site
Luciferases
Insulin-Like Growth Factor I
Reporter Genes
Base Pairing
Plasmids
Cell Culture Techniques
Binding Sites
RNA
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Platelet-derived growth factor increases the activity of the promoter of the insulin-like growth factor-1 (IGF-1) receptor gene. / Rubini, Michele; Werner, Haim; Gandini, Enrico; Roberts, Charles; Leroith, Derek; Baserga, Renato.

In: Experimental Cell Research, Vol. 211, No. 2, 1994, p. 374-379.

Research output: Contribution to journalArticle

Rubini, Michele ; Werner, Haim ; Gandini, Enrico ; Roberts, Charles ; Leroith, Derek ; Baserga, Renato. / Platelet-derived growth factor increases the activity of the promoter of the insulin-like growth factor-1 (IGF-1) receptor gene. In: Experimental Cell Research. 1994 ; Vol. 211, No. 2. pp. 374-379.
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