Platelet-derived growth factor increases the activity of the promoter of the insulin-like growth factor-1 (IGF-1) receptor gene

Michele Rubini, Haim Werner, Enrico Gandini, Charles T. Roberts, Derek Leroith, Renato Baserga

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Stimulation by platelet-derived growth factor (PDGF) is known to increase the number of IGF-I binding sites in cells in culture. We show here that PDGF also increases the levels of IGF-1 receptor mRNA. Using cell lines stably transfected with an expression plasmid in which the reporter luciferase gene is under the control of the rat IGF-1 receptor gene promoter, we find that PDGF increases the activity of this promoter. A short IGF-1 receptor gene promoter, comprising about 100 base pairs of the sequence immediately upstream of the initiation of transcription site, is sufficient for a response to the stimulatory action of PDGF. These results suggest that an increase in RNA levels and in promoter activity may play an important role in the increase in IGF-1 receptor levels that occurs after stimulation by PDGF.

Original languageEnglish (US)
Pages (from-to)374-379
Number of pages6
JournalExperimental Cell Research
Volume211
Issue number2
DOIs
StatePublished - Apr 1994
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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