Platelet activation by a novel solid-phase agonist: Effects of VWF immobilized on polystyrene beads

Michael W. Stewart, Wai S. Etches, Lynn Boshkov, Michael J. Mant, Philip A. Gordon, Andrew R E Shaw

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The interaction between platelets stirred in suspension and VWF immobilized on polystyrene beads was studied. Platelets aggregated and released ATP in response in stirring with VWF beads. Closer examination of the interaction using transmission electron microscopy revealed that the platelets did not simply aggregate with one another but initially adhered to the beads and spread. Platelets in suspension then bound to the bead-adherent platelets forming layers of platelets associated with each bead. The VWF bead-induced platelet activation was completely inhibited by addition of monoclonal antibody (mAb) to GPIb or GPIIb/IIIa. In addition, the activation response was inhibited in the presence of aspirin, indomethacin or the thromboxane receptor antagonist BM13.177, demonstrating a dependence on an intact cyclo-oxygenase pathway. Platelet function studies were carried out on 30 patients with a history of mild bleeding using conventional optical aggregation and VWF bead-induced platelet activation. 12 patients were abnormal by conventional optical aggregometry, whereas 27 patients showed depressed ATP release in response to VWF beads. The results suggest that easily-bruised patients may have a platelet function defect rather than a vascular-based abnormality and that VWF bead-induced platelet activation is a more sensitive test for detecting platelet dysfunction.

Original languageEnglish (US)
Pages (from-to)321-329
Number of pages9
JournalBritish Journal of Haematology
Volume97
Issue number2
StatePublished - 1997

Fingerprint

Polystyrenes
Platelet Activation
Blood Platelets
Suspensions
Adenosine Triphosphate
Thromboxane Receptors
Prostaglandin-Endoperoxide Synthases
Transmission Electron Microscopy
Indomethacin
Aspirin
Blood Vessels
Monoclonal Antibodies
Hemorrhage

Keywords

  • adhesion
  • aggregation
  • microparticle
  • platelet function
  • von Willebrand factor

ASJC Scopus subject areas

  • Hematology

Cite this

Stewart, M. W., Etches, W. S., Boshkov, L., Mant, M. J., Gordon, P. A., & Shaw, A. R. E. (1997). Platelet activation by a novel solid-phase agonist: Effects of VWF immobilized on polystyrene beads. British Journal of Haematology, 97(2), 321-329.

Platelet activation by a novel solid-phase agonist : Effects of VWF immobilized on polystyrene beads. / Stewart, Michael W.; Etches, Wai S.; Boshkov, Lynn; Mant, Michael J.; Gordon, Philip A.; Shaw, Andrew R E.

In: British Journal of Haematology, Vol. 97, No. 2, 1997, p. 321-329.

Research output: Contribution to journalArticle

Stewart, MW, Etches, WS, Boshkov, L, Mant, MJ, Gordon, PA & Shaw, ARE 1997, 'Platelet activation by a novel solid-phase agonist: Effects of VWF immobilized on polystyrene beads', British Journal of Haematology, vol. 97, no. 2, pp. 321-329.
Stewart MW, Etches WS, Boshkov L, Mant MJ, Gordon PA, Shaw ARE. Platelet activation by a novel solid-phase agonist: Effects of VWF immobilized on polystyrene beads. British Journal of Haematology. 1997;97(2):321-329.
Stewart, Michael W. ; Etches, Wai S. ; Boshkov, Lynn ; Mant, Michael J. ; Gordon, Philip A. ; Shaw, Andrew R E. / Platelet activation by a novel solid-phase agonist : Effects of VWF immobilized on polystyrene beads. In: British Journal of Haematology. 1997 ; Vol. 97, No. 2. pp. 321-329.
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