Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles

Rachel A. Dumont, Ulysses Lins, Adelaida G. Filoteo, John T. Penniston, Bechara Kachar, Peter Barr-Gillespie

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Mechanoelectrical transduction channels of hair cells allow for the entry of appreciable amounts of Ca2+, which regulates adaptation and triggers the mechanical activity of hair bundles. Most Ca2+ that enters transduction channels is extruded by the plasma membrane Ca2+-ATPase (PMCA), a Ca2+ pump that is highly concentrated in hair bundles and may be essential for normal hair cell function. Because PMCA isozymes and splice forms are regulated differentially and have distinct biochemical properties, we determined the identity of hair bundle PMCA in frog and rat hair cells. By screening a bullfrog saccular cDNA library, we identified abundant PMCA1 b and PMCA2a clones as well as rare PMCA2b and PMCA2c clones. Using immunocytochemistry and immunoprecipitation experiments, we showed in bullfrog sacculus that PMCA1b is the major isozyme of hair cell and supporting cell basolateral membranes and that PMCA2a is the only PMCA present in hair bundles. This complete segregation of PMCA1 and PMCA2 isozymes holds for rat auditory and vestibular hair cells; PMCA2a is the only PMCA isoform in hair bundles of outer hair cells and vestibular hair cells and is the predominant PMCA of hair bundles of inner hair cells. Our data suggest that hair cells control plasma membrane Ca2+-pumping activity by targeting specific PMCA isozymes to distinct subcellular locations. Because PMCA2a is the only Ca2+ pump present at appreciable levels in hair bundles, the biochemical properties of this pump must account fully for the physiological features of transmembrane Ca2+ pumping in bundles.

Original languageEnglish (US)
Pages (from-to)5066-5078
Number of pages13
JournalJournal of Neuroscience
Volume21
Issue number14
StatePublished - Jul 15 2001

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Calcium-Transporting ATPases
Protein Isoforms
Cell Membrane
Isoenzymes
Vestibular Hair Cells
Rana catesbeiana
Outer Auditory Hair Cells
Inner Auditory Hair Cells
Auditory Hair Cells
Clone Cells
Gene Library
Immunoprecipitation
Anura
Immunohistochemistry

Keywords

  • Calcium
  • Calcium pump
  • Cochlea
  • Hair cells
  • Isozymes
  • Stereocilia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dumont, R. A., Lins, U., Filoteo, A. G., Penniston, J. T., Kachar, B., & Barr-Gillespie, P. (2001). Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles. Journal of Neuroscience, 21(14), 5066-5078.

Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles. / Dumont, Rachel A.; Lins, Ulysses; Filoteo, Adelaida G.; Penniston, John T.; Kachar, Bechara; Barr-Gillespie, Peter.

In: Journal of Neuroscience, Vol. 21, No. 14, 15.07.2001, p. 5066-5078.

Research output: Contribution to journalArticle

Dumont, RA, Lins, U, Filoteo, AG, Penniston, JT, Kachar, B & Barr-Gillespie, P 2001, 'Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles', Journal of Neuroscience, vol. 21, no. 14, pp. 5066-5078.
Dumont RA, Lins U, Filoteo AG, Penniston JT, Kachar B, Barr-Gillespie P. Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles. Journal of Neuroscience. 2001 Jul 15;21(14):5066-5078.
Dumont, Rachel A. ; Lins, Ulysses ; Filoteo, Adelaida G. ; Penniston, John T. ; Kachar, Bechara ; Barr-Gillespie, Peter. / Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 14. pp. 5066-5078.
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