TY - JOUR
T1 - Plasma ascorbate concentrations and blood cell dehydroascorbate transport in patients with diabetes mellitus
AU - Stankova, L.
AU - Riddle, M.
AU - Larned, J.
AU - Burry, K.
AU - Menashe, D.
AU - Hart, J.
AU - Bigley, R.
N1 - Funding Information:
From thedepartments of Medical Genetics, Medicine. and Obstetrics and Gynecology, Oregon Health Sciences University. Portland, Ore. Supported by National Institutes of Health Grant Ah4 27483 and by the Oregon A#iliate, American Diabetes Association. Address reprint requests to R. Bigiey, MD L103, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd. Portland, OR 97201. 0 I984 by Grune & Stratton, Inc. 0026&0495/84/3304-001 1%01.00/O
PY - 1984/4
Y1 - 1984/4
N2 - Rates of dehydroascorbate uptake by blood granulocytes and mononuclear cells are slower, and plasma ascorbate concentrations are lower, among persons with diabetes mellitus than in nondiabetic subjects. These measurements do not correlate with one another or with simultaneously measured plasma glucose or glycosylated hemoglobin; they do not differ with type of diabetes or mode of treatment. In those diabetic granulocytes that exhibit slow dehydroascorbate uptake, maximal velocity (Vmax) transport rates for dehydroascorbate, 2-deoxyglucose, and 3-O-methylglucose are decreased, each to the same degree, while Km values for transport of these ligands are not different from those observed in nondiabetic cells. Since diffusion of these ligands is facilitated by a common transporter, these observations may reflect decreased numbers of glucose transporters in the plasma membranes of some diabetic leukocytes.
AB - Rates of dehydroascorbate uptake by blood granulocytes and mononuclear cells are slower, and plasma ascorbate concentrations are lower, among persons with diabetes mellitus than in nondiabetic subjects. These measurements do not correlate with one another or with simultaneously measured plasma glucose or glycosylated hemoglobin; they do not differ with type of diabetes or mode of treatment. In those diabetic granulocytes that exhibit slow dehydroascorbate uptake, maximal velocity (Vmax) transport rates for dehydroascorbate, 2-deoxyglucose, and 3-O-methylglucose are decreased, each to the same degree, while Km values for transport of these ligands are not different from those observed in nondiabetic cells. Since diffusion of these ligands is facilitated by a common transporter, these observations may reflect decreased numbers of glucose transporters in the plasma membranes of some diabetic leukocytes.
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U2 - 10.1016/0026-0495(84)90197-5
DO - 10.1016/0026-0495(84)90197-5
M3 - Article
C2 - 6708818
AN - SCOPUS:0021250242
SN - 0026-0495
VL - 33
SP - 347
EP - 353
JO - Metabolism
JF - Metabolism
IS - 4
ER -