Placental expression of erythropoietin mRNA, protein and receptor in sheep

M. J. Kim, L. Bogic, Cecilia Cheung, R. A. Brace

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In ovine placentae at 100 days gestation, we localized the expression of erythropoietin (EPO) mRNA by in situ hybridization and determined the cellular localization of EPO protein and EPO receptor protein by fluorescence immunohistochemistry. Erythropoietin mRNA was observed in maternal tissue at the apical tips of the fetal cytotrophoblastic villi at their interface with the maternal caruncle and was absent from both the centrally located fetal-maternal tissue and the more peripherally located maternal caruncle. An EPO-protein-associated fluorescent signal was observed in the same interface region as the EPO mRNA hybridization signal. An intense fluorescent signal associated with EPO receptor protein was observed in the apical fetal-maternal interface region with a lower density signal dispersed throughout the remainder of the interdigitating fetal-maternal tissue. The predominant cells in the apical fetal-maternal interface were identified as binucleate cells by immunohistochemistry. Thus the localization of the binucleate cells was the same as that for the EPO mRNA and the EPO protein, whereas the EPO receptor had a more generalized distribution. Since the binucleate cells are hormone producing cells, we speculate that the binucleate cells are the source of the EPO that is present in ovine placenta.

Original languageEnglish (US)
Pages (from-to)484-489
Number of pages6
JournalPlacenta
Volume22
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

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Erythropoietin
Sheep
Mothers
Messenger RNA
Erythropoietin Receptors
Proteins
Placenta
Fetus
Immunohistochemistry
In Situ Hybridization
Fluorescence
Hormones
Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Placental expression of erythropoietin mRNA, protein and receptor in sheep. / Kim, M. J.; Bogic, L.; Cheung, Cecilia; Brace, R. A.

In: Placenta, Vol. 22, No. 5, 2001, p. 484-489.

Research output: Contribution to journalArticle

Kim, MJ, Bogic, L, Cheung, C & Brace, RA 2001, 'Placental expression of erythropoietin mRNA, protein and receptor in sheep', Placenta, vol. 22, no. 5, pp. 484-489. https://doi.org/10.1053/plac.2001.0681
Kim, M. J. ; Bogic, L. ; Cheung, Cecilia ; Brace, R. A. / Placental expression of erythropoietin mRNA, protein and receptor in sheep. In: Placenta. 2001 ; Vol. 22, No. 5. pp. 484-489.
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