PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae

Matthew Wolfgang, Peter Lauer, Haesun Park, Laurent Brossay, Jacques Hébert, Michael Koomey

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

Neisseria gonorrhoeae, the Gram-negative aetiological agent or gonorrhoea, is one of many mucosal pathogenes of man that expresses competence for natural transformation. Expression of this phenotype by gonococci appears to rely on the expression of type IV pili (Tip), but the mechanistic basis for this relationship remains unknown. During studies of gonococcal plus biogenesis, a homologue on the PilT family of proteins, required for Tip-dependent twitching motility in Pseudomonas aeruginosa and social gliding motility in Mytococcus xanthus, was discovered. Like the findings in these other species, we show here that gonococcal pilT mutants constructed in vitro no longer display twitching motility. In addition, we demonstrate that they have concurrently lost the ability to undergo natural transformation, despite the expression of structurally and morphologically normal Tfp. These results were confirmed by the findings that two classes of spontaneous mutants that failed to express twitching motility and transformability carried mutations in pilT. Piliated pilT mutants and a panel of pilus assembly mutants were found to be deficient in sequence-specific DNA uptake into the cell, the earliest demonstrable step in neisserial competence. The PilT-deficient strains represent the first genetically defined mutants that are defective in DNA uptake but retain Tfp expression.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalMolecular Microbiology
Volume29
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

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Neisseria gonorrhoeae
Mental Competency
Mutation
Aptitude
Gonorrhea
Pseudomonas aeruginosa
Phenotype
DNA
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae. / Wolfgang, Matthew; Lauer, Peter; Park, Haesun; Brossay, Laurent; Hébert, Jacques; Koomey, Michael.

In: Molecular Microbiology, Vol. 29, No. 1, 1998, p. 321-330.

Research output: Contribution to journalArticle

Wolfgang, Matthew ; Lauer, Peter ; Park, Haesun ; Brossay, Laurent ; Hébert, Jacques ; Koomey, Michael. / PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae. In: Molecular Microbiology. 1998 ; Vol. 29, No. 1. pp. 321-330.
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