Piezo1 ion channel pore properties are dictated by C-terminal region

Bertrand Coste, Swetha E. Murthy, Jayanti Mathur, Manuela Schmidt, Yasmine Mechioukhi, Patrick Delmas, Ardem Patapoutian

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

Piezo1 and Piezo2 encode mechanically activated cation channels that function as mechanotransducers involved in vascular system development and touch sensing, respectively. Structural features of Piezos remain unknown. Mouse Piezo1 is bioinformatically predicted to have 30-40 transmembrane (TM) domains. Here, we find that nine of the putative inter-transmembrane regions are accessible from the extracellular side. We use chimeras between mPiezo1 and dPiezo to show that ion-permeation properties are conferred by C-terminal region. We further identify a glutamate residue within a conserved region adjacent to the last two putative TM domains of the protein, that when mutated, affects unitary conductance and ion selectivity, and modulates pore block. We propose that this amino acid is either in the pore or closely associates with the pore. Our results describe important structural motifs of this channel family and lay the groundwork for a mechanistic understanding of how Piezos are mechanically gated and conduct ions.

Original languageEnglish (US)
Article number7223
JournalNature communications
Volume6
DOIs
StatePublished - May 26 2015
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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