PI3K signaling in the murine kidney inner medullary cell response to urea

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Growth factors and other stimuli increase the activity of phosphatidylinositol-3 kinase (PI3K), an SH2 domain-containing lipid kinase. In the murine kidney inner medullary mIMCD3 cell line, urea (200 mM) increased PI3K activity in a time-dependent fashion as measured by immune complex kinase assay. The PI3K effector, Akt, was also activated by urea as measured by antiphospho-Akt immunoblotting. In addition, the Akt (and PI3K) effector, p70 S6 kinase, was activated by urea treatment in a PI3K-dependent fashion. PI3K inhibition potentiated the proapoptotic effect of hypertonic and urea stress. Urea treatment also induced the tyrosine phosphorylation of She and the recruitment to She of Grb2. Coexistence of activated She and PI3K in a macromolecular complex was suggested by the increase in PI3K activity evident in anti-Shc immunoprecipitates prepared from urea-treated cells. Taken together, these data suggest that PI3K may regulate physiological events in the renal medullary cell response to urea stress and that an upstream tyrosine kinase conferring activation of both PI3K and She may govern urea signaling in these cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume278
Issue number1
StatePublished - 2000

Fingerprint

Phosphatidylinositol 3-Kinase
Urea
Kidney
Phosphotransferases
70-kDa Ribosomal Protein S6 Kinases
Macromolecular Substances
src Homology Domains
Osmotic Pressure
Antigen-Antibody Complex
Immunoblotting
Protein-Tyrosine Kinases
Tyrosine
Intercellular Signaling Peptides and Proteins
Phosphorylation

Keywords

  • Apoptosis
  • Hypertonicity
  • MIMCD3 cells
  • Sodium chloride
  • Stress

ASJC Scopus subject areas

  • Physiology

Cite this

@article{0556e9c4c367474f9a65f79d41bcef43,
title = "PI3K signaling in the murine kidney inner medullary cell response to urea",
abstract = "Growth factors and other stimuli increase the activity of phosphatidylinositol-3 kinase (PI3K), an SH2 domain-containing lipid kinase. In the murine kidney inner medullary mIMCD3 cell line, urea (200 mM) increased PI3K activity in a time-dependent fashion as measured by immune complex kinase assay. The PI3K effector, Akt, was also activated by urea as measured by antiphospho-Akt immunoblotting. In addition, the Akt (and PI3K) effector, p70 S6 kinase, was activated by urea treatment in a PI3K-dependent fashion. PI3K inhibition potentiated the proapoptotic effect of hypertonic and urea stress. Urea treatment also induced the tyrosine phosphorylation of She and the recruitment to She of Grb2. Coexistence of activated She and PI3K in a macromolecular complex was suggested by the increase in PI3K activity evident in anti-Shc immunoprecipitates prepared from urea-treated cells. Taken together, these data suggest that PI3K may regulate physiological events in the renal medullary cell response to urea stress and that an upstream tyrosine kinase conferring activation of both PI3K and She may govern urea signaling in these cells.",
keywords = "Apoptosis, Hypertonicity, MIMCD3 cells, Sodium chloride, Stress",
author = "David Cohen",
year = "2000",
language = "English (US)",
volume = "278",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - PI3K signaling in the murine kidney inner medullary cell response to urea

AU - Cohen, David

PY - 2000

Y1 - 2000

N2 - Growth factors and other stimuli increase the activity of phosphatidylinositol-3 kinase (PI3K), an SH2 domain-containing lipid kinase. In the murine kidney inner medullary mIMCD3 cell line, urea (200 mM) increased PI3K activity in a time-dependent fashion as measured by immune complex kinase assay. The PI3K effector, Akt, was also activated by urea as measured by antiphospho-Akt immunoblotting. In addition, the Akt (and PI3K) effector, p70 S6 kinase, was activated by urea treatment in a PI3K-dependent fashion. PI3K inhibition potentiated the proapoptotic effect of hypertonic and urea stress. Urea treatment also induced the tyrosine phosphorylation of She and the recruitment to She of Grb2. Coexistence of activated She and PI3K in a macromolecular complex was suggested by the increase in PI3K activity evident in anti-Shc immunoprecipitates prepared from urea-treated cells. Taken together, these data suggest that PI3K may regulate physiological events in the renal medullary cell response to urea stress and that an upstream tyrosine kinase conferring activation of both PI3K and She may govern urea signaling in these cells.

AB - Growth factors and other stimuli increase the activity of phosphatidylinositol-3 kinase (PI3K), an SH2 domain-containing lipid kinase. In the murine kidney inner medullary mIMCD3 cell line, urea (200 mM) increased PI3K activity in a time-dependent fashion as measured by immune complex kinase assay. The PI3K effector, Akt, was also activated by urea as measured by antiphospho-Akt immunoblotting. In addition, the Akt (and PI3K) effector, p70 S6 kinase, was activated by urea treatment in a PI3K-dependent fashion. PI3K inhibition potentiated the proapoptotic effect of hypertonic and urea stress. Urea treatment also induced the tyrosine phosphorylation of She and the recruitment to She of Grb2. Coexistence of activated She and PI3K in a macromolecular complex was suggested by the increase in PI3K activity evident in anti-Shc immunoprecipitates prepared from urea-treated cells. Taken together, these data suggest that PI3K may regulate physiological events in the renal medullary cell response to urea stress and that an upstream tyrosine kinase conferring activation of both PI3K and She may govern urea signaling in these cells.

KW - Apoptosis

KW - Hypertonicity

KW - MIMCD3 cells

KW - Sodium chloride

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=20444420936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444420936&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:20444420936

VL - 278

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 1

ER -