Physiological recordings from zebrafish lateral-line hair cells and afferent neurons

Josef G. Trapani, Teresa Nicolson

Research output: Book/ReportBook

41 Citations (Scopus)

Abstract

Sensory signal transduction, the process by which the features of external stimuli are encoded into action potentials, is a complex process that is not fully understood. In fish and amphibia, the lateral-line organ detects water movement and vibration and is critical for schooling behavior and the detection of predators and prey. The lateral-line system in zebrafish serves as an ideal platform to examine encoding of stimuli by sensory hair cells. Here, we describe methods for recording hair-cell microphonics and activity of afferent neurons using intact zebrafish larvae. The recordings are performed by immobilizing and mounting larvae for optimal stimulation of lateral-line hair cells. Hair cells are stimulated with a pressure-controlled water jet and a recording electrode is positioned next to the site of mechanotransduction in order to record microphonics-extracellular voltage changes due to currents through hair-cell mechanotransduction channels. Another readout of the hair-cell activity is obtained by recording action currents from single afferent neurons in response to water-jet stimulation of innervated hair cells. When combined, these techniques make it possible to probe the function of the lateral-line sensory system in an intact zebrafish using controlled, repeatable, physiological stimuli.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages13
Volume100
EditionC
DOIs
StatePublished - 2010

Publication series

NameMethods in Cell Biology
No.C
Volume100
ISSN (Print)0091679X

Fingerprint

Afferent Neurons
Zebrafish
Lateral Line System
Larva
Water Movements
Water
Amphibians
Vibration
Action Potentials
Signal Transduction
Electrodes
Fishes
Pressure

Keywords

  • Action currents
  • Afferent neuron
  • Auditory
  • Hair cell
  • Lateral-line
  • Mechanotransduction
  • Neuromast
  • Sensory system

ASJC Scopus subject areas

  • Cell Biology

Cite this

Physiological recordings from zebrafish lateral-line hair cells and afferent neurons. / Trapani, Josef G.; Nicolson, Teresa.

C ed. Unknown Publisher, 2010. 13 p. (Methods in Cell Biology; Vol. 100, No. C).

Research output: Book/ReportBook

Trapani, Josef G. ; Nicolson, Teresa. / Physiological recordings from zebrafish lateral-line hair cells and afferent neurons. C ed. Unknown Publisher, 2010. 13 p. (Methods in Cell Biology; C).
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