Physical mapping of human chromosome 17 using fragment-containing microcell hybrids

Robin J. Leach, Mathew (Matt) Thayer, Alan J. Schafer, R. E K Fournier

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Hybrid cell lines were generated by microcell-mediated transfer of human chromosome 17 into rat recipient cells. The genotypes of 36 such lines were analyzed using a set of human chromosome 17-derived sequences to probe the structural integrity of the chromosome. Four classes of hybrids were obtained: clones with an apparently intact chromosome 17, clones containing large fragments of the chromosome including both the centromere and the selected marker, clones containing only the selected marker and flanking sequences, and clones containing two 17-derived fragments-the pericentric region plus the region of the selected marker. Data from these hybrids were used in conjunction with published regional localization information to obtain a provisional linear map of the chromosome. Results of this analysis are compared to the gene maps predicted from recent linkage studies and from other somatic cell hybrid experiments.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalGenomics
Volume5
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

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Chromosomes, Human, Pair 17
Human Chromosomes
Clone Cells
Hybrid Cells
Chromosomes
Centromere
Genotype
Cell Line
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. / Leach, Robin J.; Thayer, Mathew (Matt); Schafer, Alan J.; Fournier, R. E K.

In: Genomics, Vol. 5, No. 2, 1989, p. 167-176.

Research output: Contribution to journalArticle

Leach, Robin J. ; Thayer, Mathew (Matt) ; Schafer, Alan J. ; Fournier, R. E K. / Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. In: Genomics. 1989 ; Vol. 5, No. 2. pp. 167-176.
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