Physical mapping of human chromosome 17 using fragment-containing microcell hybrids

Robin J. Leach; Mathew J. Thayer; Alan J. Schafer; R. E.K. Fournier

doi:10.1016/0888-7543(89)90043-8

Physical mapping of human chromosome 17 using fragment-containing microcell hybrids

Robin J. Leach, Mathew J. Thayer, Alan J. Schafer, R. E.K. Fournier

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Hybrid cell lines were generated by microcell-mediated transfer of human chromosome 17 into rat recipient cells. The genotypes of 36 such lines were analyzed using a set of human chromosome 17-derived sequences to probe the structural integrity of the chromosome. Four classes of hybrids were obtained: clones with an apparently intact chromosome 17, clones containing large fragments of the chromosome including both the centromere and the selected marker, clones containing only the selected marker and flanking sequences, and clones containing two 17-derived fragments-the pericentric region plus the region of the selected marker. Data from these hybrids were used in conjunction with published regional localization information to obtain a provisional linear map of the chromosome. Results of this analysis are compared to the gene maps predicted from recent linkage studies and from other somatic cell hybrid experiments.

Original language	English (US)
Pages (from-to)	167-176
Number of pages	10
Journal	Genomics
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/0888-7543(89)90043-8
State	Published - Aug 1989
Externally published	Yes

ASJC Scopus subject areas

Genetics

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@article{21fd2d9baa4143248477e0f8fe753233,

title = "Physical mapping of human chromosome 17 using fragment-containing microcell hybrids",

abstract = "Hybrid cell lines were generated by microcell-mediated transfer of human chromosome 17 into rat recipient cells. The genotypes of 36 such lines were analyzed using a set of human chromosome 17-derived sequences to probe the structural integrity of the chromosome. Four classes of hybrids were obtained: clones with an apparently intact chromosome 17, clones containing large fragments of the chromosome including both the centromere and the selected marker, clones containing only the selected marker and flanking sequences, and clones containing two 17-derived fragments-the pericentric region plus the region of the selected marker. Data from these hybrids were used in conjunction with published regional localization information to obtain a provisional linear map of the chromosome. Results of this analysis are compared to the gene maps predicted from recent linkage studies and from other somatic cell hybrid experiments.",

author = "Leach, {Robin J.} and Thayer, {Mathew J.} and Schafer, {Alan J.} and Fournier, {R. E.K.}",

note = "Funding Information: We thank the following individuals for providing recombinant plasmids-L. Chandler, M. Chao, J. Corden. R. Gelinas, C. Larson, L. Leinwand, F. Ramirez, F. Ruddle, and A. Ullrich. We thank P. O{\textquoteright}Connell and R. White for providing the “DS” plasmids, and H. Willard for D17Zl. These studies were supported by Grant GM26449 from the National Institute of General Medical Sciences. R.J.L. was supported by postdoctoral fellowships from the Leukemia Society of America and from the NIH. R.E.K.F. is the recipient of American Cancer Society Faculty Research Award.",

year = "1989",

month = aug,

doi = "10.1016/0888-7543(89)90043-8",

language = "English (US)",

volume = "5",

pages = "167--176",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Physical mapping of human chromosome 17 using fragment-containing microcell hybrids

AU - Leach, Robin J.

AU - Thayer, Mathew J.

AU - Schafer, Alan J.

AU - Fournier, R. E.K.

N1 - Funding Information: We thank the following individuals for providing recombinant plasmids-L. Chandler, M. Chao, J. Corden. R. Gelinas, C. Larson, L. Leinwand, F. Ramirez, F. Ruddle, and A. Ullrich. We thank P. O’Connell and R. White for providing the “DS” plasmids, and H. Willard for D17Zl. These studies were supported by Grant GM26449 from the National Institute of General Medical Sciences. R.J.L. was supported by postdoctoral fellowships from the Leukemia Society of America and from the NIH. R.E.K.F. is the recipient of American Cancer Society Faculty Research Award.

PY - 1989/8

Y1 - 1989/8

N2 - Hybrid cell lines were generated by microcell-mediated transfer of human chromosome 17 into rat recipient cells. The genotypes of 36 such lines were analyzed using a set of human chromosome 17-derived sequences to probe the structural integrity of the chromosome. Four classes of hybrids were obtained: clones with an apparently intact chromosome 17, clones containing large fragments of the chromosome including both the centromere and the selected marker, clones containing only the selected marker and flanking sequences, and clones containing two 17-derived fragments-the pericentric region plus the region of the selected marker. Data from these hybrids were used in conjunction with published regional localization information to obtain a provisional linear map of the chromosome. Results of this analysis are compared to the gene maps predicted from recent linkage studies and from other somatic cell hybrid experiments.

AB - Hybrid cell lines were generated by microcell-mediated transfer of human chromosome 17 into rat recipient cells. The genotypes of 36 such lines were analyzed using a set of human chromosome 17-derived sequences to probe the structural integrity of the chromosome. Four classes of hybrids were obtained: clones with an apparently intact chromosome 17, clones containing large fragments of the chromosome including both the centromere and the selected marker, clones containing only the selected marker and flanking sequences, and clones containing two 17-derived fragments-the pericentric region plus the region of the selected marker. Data from these hybrids were used in conjunction with published regional localization information to obtain a provisional linear map of the chromosome. Results of this analysis are compared to the gene maps predicted from recent linkage studies and from other somatic cell hybrid experiments.

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VL - 5

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JO - Genomics

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Physical mapping of human chromosome 17 using fragment-containing microcell hybrids

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