Physical mapping of chromosome 17 cosmids by fluorescence in situ hybridization and digital image analysis

O. P. Kallioniemi, A. Kallioniemi, L. Mascio, D. Sudar, D. Pinkel, L. Deaven, J. Gray

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

We used fluorescence in situ hybridization and digital image analysis to localize cosmids along human chromosome 17. Seventy-one cosmids were selected at random from a chromosome 17 library constructed from a partial Sau3 AI digest of flow-sorted chromosomes from a mouse-human hybrid cell line. Sixty-three of these (89%) gave a signal only on chromosome 17. The 40 cosmids producing the most distinct hybridization signals in metaphase and interphase cells were precisely mapped using digital image analysis. An additional 20 cosmids, previously mapped by linkage analysis, were also mapped. The order of these probes determined by metaphase mapping was consistent with the order determined by linkage analysis.

Original languageEnglish (US)
Pages (from-to)125-128
Number of pages4
JournalGenomics
Volume20
Issue number1
DOIs
StatePublished - Mar 1 1994

ASJC Scopus subject areas

  • Genetics

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    Kallioniemi, O. P., Kallioniemi, A., Mascio, L., Sudar, D., Pinkel, D., Deaven, L., & Gray, J. (1994). Physical mapping of chromosome 17 cosmids by fluorescence in situ hybridization and digital image analysis. Genomics, 20(1), 125-128. https://doi.org/10.1006/geno.1994.1138