Physical characteristics of immunore active chorionic gonadotropin produced in culture

J. M. Hammond, W. E. Bridson, P. O. Kohler, A. Chrambach

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Immunoreactive chorionic gonadotropin (CG) produced by human choriocarcinoma cells in culture was compared with CG from urinary sources by polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing in polyacrylamide gel (IFPA). CG from both sources had the same molecular size in PAGE operative at pH 10.2 and 5.5. However, PAGE revealed heterogeneity with regard to molecular net charge for both hormone preparations. Two species of CG characterized by pis of 3 and 4.5 were re vealed in the tissue culture medium on examination by IFPA. Digestion of both species with neuraminidase produced a single immunoreactive CG with pi of 6. Heterogeneity of CG with regard to net charge and isoelectric point may reflect variable completion of the carbohydrate portion of these molecules at the cell of origin.

Original languageEnglish (US)
Pages (from-to)801-806
Number of pages6
JournalEndocrinology
Volume89
Issue number3
DOIs
StatePublished - Sep 1971

ASJC Scopus subject areas

  • Endocrinology

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