Photopolymerization of cell-laden gelatin methacryloyl hydrogels using a dental curing light for regenerative dentistry

Nelson Monteiro, Greeshma Thrivikraman, Avathamsa Athirasala, Anthony Tahayeri, Cristiane M. França, Jack Ferracane, Luiz Bertassoni

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Photopolymerized hydrogels, such as gelatin methacryloyl (GelMA), have desirable biological and mechanical characteristics for a range of tissue engineering applications. Objective: This study aimed to optimize a new method to photopolymerize GelMA using a dental curing light (DL). Methods: Lithium acylphosphinate photo-initiator (LAP, 0.05, 0.067, 0.1% w/v) was evaluated for its ability to polymerize GelMA hydrogel precursors (10% w/v) encapsulated with odontoblast-like cells (OD21). Different irradiances (1650, 2300 and 3700mW/cm2) and photo-curing times (5-20s) were tested, and compared against the parameters typically used in UV light photopolymerization (45mW/cm2, 0.1% w/v Irgacure 2959 as photoinitiator). Physical and mechanical properties of the photopolymerized GelMA hydrogels were determined. Cell viability was assessed using a live and dead assay kit. Results: Comparing DL and UV polymerization methods, the DL method photopolymerized GelMA precursor faster and presented larger pore size than the UV polymerization method. The live and dead assay showed more than 80% of cells were viable when hydrogels were photopolymerized with the different DL irradiances. However, the cell viability decreased when the exposure time was increased to 20s using the 1650mW/cm2 intensity, and when the LAP concentration was increased from 0.05 to 0.1%. Both DL and UV photocrosslinked hydrogels supported a high percentage of cell viability and enabled fabrication of micropatterns using a photolithography microfabrication technique. Significance: The proposed method to photopolymerize GelMA cell-laden hydrogels using a dental curing light is effective and represents an important step towards the establishment of chair-side procedures in regenerative dentistry.

Original languageEnglish (US)
JournalDental Materials
DOIs
StateAccepted/In press - Jan 1 2017

Fingerprint

Dental Curing Lights
Dentistry
Hydrogels
Photopolymerization
Gelatin
Curing
Cell Survival
Cells
Polymerization
Assays
Microtechnology
Odontoblasts
Hydrogel
Microfabrication
Photolithography
Tissue Engineering
Ultraviolet Rays
Lithium
Tissue engineering
Ultraviolet radiation

Keywords

  • Bioengineering
  • Biomedical and dental materials
  • Endodontics
  • Hydrogel
  • Odontoblast
  • Regenerative medicine
  • Visible light

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Photopolymerization of cell-laden gelatin methacryloyl hydrogels using a dental curing light for regenerative dentistry. / Monteiro, Nelson; Thrivikraman, Greeshma; Athirasala, Avathamsa; Tahayeri, Anthony; França, Cristiane M.; Ferracane, Jack; Bertassoni, Luiz.

In: Dental Materials, 01.01.2017.

Research output: Contribution to journalArticle

Monteiro, Nelson ; Thrivikraman, Greeshma ; Athirasala, Avathamsa ; Tahayeri, Anthony ; França, Cristiane M. ; Ferracane, Jack ; Bertassoni, Luiz. / Photopolymerization of cell-laden gelatin methacryloyl hydrogels using a dental curing light for regenerative dentistry. In: Dental Materials. 2017.
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abstract = "Photopolymerized hydrogels, such as gelatin methacryloyl (GelMA), have desirable biological and mechanical characteristics for a range of tissue engineering applications. Objective: This study aimed to optimize a new method to photopolymerize GelMA using a dental curing light (DL). Methods: Lithium acylphosphinate photo-initiator (LAP, 0.05, 0.067, 0.1{\%} w/v) was evaluated for its ability to polymerize GelMA hydrogel precursors (10{\%} w/v) encapsulated with odontoblast-like cells (OD21). Different irradiances (1650, 2300 and 3700mW/cm2) and photo-curing times (5-20s) were tested, and compared against the parameters typically used in UV light photopolymerization (45mW/cm2, 0.1{\%} w/v Irgacure 2959 as photoinitiator). Physical and mechanical properties of the photopolymerized GelMA hydrogels were determined. Cell viability was assessed using a live and dead assay kit. Results: Comparing DL and UV polymerization methods, the DL method photopolymerized GelMA precursor faster and presented larger pore size than the UV polymerization method. The live and dead assay showed more than 80{\%} of cells were viable when hydrogels were photopolymerized with the different DL irradiances. However, the cell viability decreased when the exposure time was increased to 20s using the 1650mW/cm2 intensity, and when the LAP concentration was increased from 0.05 to 0.1{\%}. Both DL and UV photocrosslinked hydrogels supported a high percentage of cell viability and enabled fabrication of micropatterns using a photolithography microfabrication technique. Significance: The proposed method to photopolymerize GelMA cell-laden hydrogels using a dental curing light is effective and represents an important step towards the establishment of chair-side procedures in regenerative dentistry.",
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AU - Thrivikraman, Greeshma

AU - Athirasala, Avathamsa

AU - Tahayeri, Anthony

AU - França, Cristiane M.

AU - Ferracane, Jack

AU - Bertassoni, Luiz

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AB - Photopolymerized hydrogels, such as gelatin methacryloyl (GelMA), have desirable biological and mechanical characteristics for a range of tissue engineering applications. Objective: This study aimed to optimize a new method to photopolymerize GelMA using a dental curing light (DL). Methods: Lithium acylphosphinate photo-initiator (LAP, 0.05, 0.067, 0.1% w/v) was evaluated for its ability to polymerize GelMA hydrogel precursors (10% w/v) encapsulated with odontoblast-like cells (OD21). Different irradiances (1650, 2300 and 3700mW/cm2) and photo-curing times (5-20s) were tested, and compared against the parameters typically used in UV light photopolymerization (45mW/cm2, 0.1% w/v Irgacure 2959 as photoinitiator). Physical and mechanical properties of the photopolymerized GelMA hydrogels were determined. Cell viability was assessed using a live and dead assay kit. Results: Comparing DL and UV polymerization methods, the DL method photopolymerized GelMA precursor faster and presented larger pore size than the UV polymerization method. The live and dead assay showed more than 80% of cells were viable when hydrogels were photopolymerized with the different DL irradiances. However, the cell viability decreased when the exposure time was increased to 20s using the 1650mW/cm2 intensity, and when the LAP concentration was increased from 0.05 to 0.1%. Both DL and UV photocrosslinked hydrogels supported a high percentage of cell viability and enabled fabrication of micropatterns using a photolithography microfabrication technique. Significance: The proposed method to photopolymerize GelMA cell-laden hydrogels using a dental curing light is effective and represents an important step towards the establishment of chair-side procedures in regenerative dentistry.

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KW - Visible light

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