Photoelectrophoretic transport and hybridization of DNA oligonucleotides on unpatterned silicon substrates

Christian Gurtner, Carl F. Edman, Rachel E. Formosa, Michael J. Heller

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We describe a platform for photoelectrophoretic transport and electronic hybridization of fluorescence-labeled DNA oligonucleotides in a low conductivity electrolyte. At the core of the platform is a chemically stabilized semiconductor photodiode or photoconductor surface coated with a streptavidin - agarose permeation layer. Micro-illumination of this surface generates photoelectrochemical currents that are used to electrophoretically transport and attach biotinylated DNA capture strands to arbitrarily selected locations. The same process is then used to transport and electronically hybridize fluorescence-labeled DNA target strands to the previously attached capture strands. Signal detection is accomplished either by a fluorescence scanner or a CCD camera. This represents a flexible electronic DNA assay platform that does not rely on pre-patterned microelectronic arrays.

Original languageEnglish (US)
Pages (from-to)8589-8594
Number of pages6
JournalJournal of the American Chemical Society
Volume122
Issue number36
DOIs
StatePublished - Sep 13 2000

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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