Photodynamic therapy with chloroaluminum sulfonated phthalocyanine in the rat window chamber

Scott J. Stern, Stephen T. Flock, Susan Small, Sharon Thomsen, Steven Jacques

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The efficacy of photodynamic therapy tumor destruction is dependent upon both the interruption of the tumor vasculature and the resultant production of unstable oxygen species causing cellular oxidation and death. Chloroaluminum sulfonated phthalocyanine (CASP) is a recently developed photosensitizer. In order to study the direct vascular effects of CASP on a non-tumor system, a rat window chamber was utilized. Twelve rats were implanted with the window chamber, and were divided into two groups of six. Three rats served as controls for each group (receiving light alone, CASP alone, or no treatment). The remaining 6 rats received 10 mg/kg CASP intravenously 4 days after chamber placement. Photoactivation with light was performed 24 hours after injection (power density 200 mW/cm2, irradiance 100 J/cm2, λ=675 nm). Utilizing integrating sphere measurements and image analysis, marked vascular changes in the form of initial vasospasm followed by vasoconstriction and loss of chamber neovascularization were noted in the CASP-PDT group. The control groups exhibited no significant changes. Manipulation of the chamber vasculature at strategic time-points may translate into improved response rates for photodynamic therapy in a tumor model.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalThe American Journal of Surgery
Volume160
Issue number4
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Photochemotherapy
Blood Vessels
Light
Neoplasms
Control Groups
Photosensitizing Agents
Vasoconstriction
chloroaluminum phthalocyanine
Oxygen
Injections

ASJC Scopus subject areas

  • Surgery

Cite this

Photodynamic therapy with chloroaluminum sulfonated phthalocyanine in the rat window chamber. / Stern, Scott J.; Flock, Stephen T.; Small, Susan; Thomsen, Sharon; Jacques, Steven.

In: The American Journal of Surgery, Vol. 160, No. 4, 1990, p. 360-364.

Research output: Contribution to journalArticle

Stern, Scott J. ; Flock, Stephen T. ; Small, Susan ; Thomsen, Sharon ; Jacques, Steven. / Photodynamic therapy with chloroaluminum sulfonated phthalocyanine in the rat window chamber. In: The American Journal of Surgery. 1990 ; Vol. 160, No. 4. pp. 360-364.
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