Photochemotherapy of intimal hyperplasia using Psoralen activated by uv light in porcine model

Lisa A. Buckley, Kenton W.M.D. Gregory, Deborah T.R.N. Bahlman, Hanqun Shangguan, Henner Fahrenbach, Eli M.D. Rosenthal, Peter C.M.D. Block

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Psoralen activated by UVA light (PUVA) was investigated as a means of inhibiting smooth muscle cell proliferation resulting from balloon injury. Twenty kilogram domestic swine were anesthetized and underwent balloon angioplasty to create a 133% overstretch injury. Assignments of treatment and control were randomized between the left anterior descending (LAD) and circumflex (LCX) coronaries arteries. The animals were given with 5 mg/kg of 8- methoxypsoralen eternally. Treatment vessels received 600 mJ/cm 2 of 364 nm light during balloon inflation to activate the psoralen. Control vessels received drug and balloon injury only. Serum was obtained during the light delivery to assess psoralen levels. At 30 days, animals were sacrificed and the coronary arteries perfusion fixed. Five sections per vessel were analyzed morphometrically to determine percent intimal area and extent of injury. The restenosis injury index was 0.21 plus or minus .02 in treatment vessels and 0.14 plus or minus .01 in the controls with a p-value less than .02. In this large animal model of balloon angioplasty injury, psoralen activated by ultraviolet light increased intimal hyperplasia.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsR.Rox Anderson
Pages78-83
Number of pages6
StatePublished - Jan 1 1996
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VI - San Jose, CA, USA
Duration: Jan 27 1996Jan 30 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2671

Other

OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VI
CitySan Jose, CA, USA
Period1/27/961/30/96

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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    Buckley, L. A., Gregory, K. W. M. D., Bahlman, D. T. R. N., Shangguan, H., Fahrenbach, H., Rosenthal, E. M. D., & Block, P. C. M. D. (1996). Photochemotherapy of intimal hyperplasia using Psoralen activated by uv light in porcine model. In R. R. Anderson (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 78-83). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2671).