Photochemical control of the infectivity of adenoviral vectors using a novel photocleavable biotinylation reagent

Mark W. Pandori, David A. Hobson, Jerzy Olejnik, Edyta Krzymanska-Olejnik, Kenneth J. Rothschild, Abraham A. Palmer, Tamara J. Phillips, Takeshi Sano

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.

Original languageEnglish (US)
Pages (from-to)567-573
Number of pages7
JournalChemistry and Biology
Volume9
Issue number5
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Photochemical control of the infectivity of adenoviral vectors using a novel photocleavable biotinylation reagent'. Together they form a unique fingerprint.

Cite this