TY - JOUR
T1 - Photochemical control of the infectivity of adenoviral vectors using a novel photocleavable biotinylation reagent
AU - Pandori, Mark W.
AU - Hobson, David A.
AU - Olejnik, Jerzy
AU - Krzymanska-Olejnik, Edyta
AU - Rothschild, Kenneth J.
AU - Palmer, Abraham A.
AU - Phillips, Tamara J.
AU - Sano, Takeshi
N1 - Funding Information:
M.W.P. was supported by a postdoctoral fellowship (PC990029) from the U.S. Department of Army Prostate Cancer Research Program. A.A.P. was supported by a training grant (AA07468) from the National Institute on Alcohol Abuse and Alcoholism, NIH. This work was supported by a grant (AI040753) from the National Institute of Allergy and Infectious Diseases, NIH, to J.O., a grant from the Department of Veterans Affairs to T.J.P., and a grant (CA46109) from the National Cancer Institute, NIH, to T.S.
PY - 2002
Y1 - 2002
N2 - We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.
AB - We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.
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U2 - 10.1016/S1074-5521(02)00135-7
DO - 10.1016/S1074-5521(02)00135-7
M3 - Article
C2 - 12031663
AN - SCOPUS:0036015839
SN - 2451-9448
VL - 9
SP - 567
EP - 573
JO - Cell Chemical Biology
JF - Cell Chemical Biology
IS - 5
ER -