Photoaffinity labeling of integrin αIIbβ3 (glycoprotein IIb-IIIa) on intact platelets with 8-azido-[γ-32P]ATP

Peter Mayinger, Meinrad Gawaz

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The fibrinogen receptor GPIIb-IIIa plays a crucial role in platelet aggregation. Here we show that the adenine nucleotide, 8-azido-ATP, inhibits ADP-induced conformational change of the platelet fibrinogen receptor GPIIb-IIIa (integrin αIIbβ3). Photoaffinity labeling of intact platelets with 8-azido[α-32P]ATP exclusively modifies two plasma-membrane glycoproteins which are identical with both subunits of GPIIb-IIIa. The presence of adenine-nucleotide-binding sites on GPIIb-IIIa implies that the platelet fibrinogen receptor is directly regulated by extracellular adenine nucleotides.

Original languageEnglish (US)
Pages (from-to)77-81
Number of pages5
JournalBBA - Molecular Cell Research
Volume1137
Issue number1
DOIs
StatePublished - Oct 6 1992

Keywords

  • 8-azido-ATP
  • GPIIb-IIIa
  • Integrin α IIbβ3
  • Nucleotide binding
  • Photoaffinity labeling
  • Platelet

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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