PHOTOACTIVABLE FLUOROPHORES FOR THE MEASUREMENT OF FLUENCE IN TURBID MEDIA

L. Lilge, T. J. Flotte, I. E. Kochevar, Steven Jacques, F. Hillenkamp

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Knowledge of the fluence distribution in biological tissue is essential for applications of lasers and light in medicine. A method using a photoactivable fluorophore as a chemical actinometer is presented to investigate the fluence (J/cm2) distribution in tissue‐simulating phantoms. Such a chemical actinometer provides high spatial resolution (≤20 μm) while minimizing the disturbance of the fluence distribution. The actinometer substance, nonfluorescent in its native state, is incorporated into an acrylamide gel. Upon absorption of 351 nm radiation (λact), the actinometer substance becomes a Ruorophorc, which is excited at λex≤ 485 nm. Thus the spatial distribution of the emitted fluorescence (λem≤ 515 nm) in the actinometer represents the fluence distribution of the activating radiation. Using histological techniques, 20 μm sections are cut from gel‐like optical phantoms containing the actinometric substance. The fluorescence intensity in the section is recorded under a standard fluorescence microscope equipped with a sensitive video camera. To simulate different biological tissues, the scattering and absorption properties of the gel phantoms arc varicd over a wide range. The experimentally obtained fluence distributions are compared with theoretical models of light distribution in turbid media.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalPhotochemistry and Photobiology
Volume58
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

actinometers
Fluorophores
fluence
Fluorescence
Gels
Radiation
Tissue
Histological Techniques
Light
Acrylamide
Video cameras
fluorescence
Spatial distribution
Medicine
Lasers
Microscopes
Theoretical Models
gels
Scattering
radiation

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

PHOTOACTIVABLE FLUOROPHORES FOR THE MEASUREMENT OF FLUENCE IN TURBID MEDIA. / Lilge, L.; Flotte, T. J.; Kochevar, I. E.; Jacques, Steven; Hillenkamp, F.

In: Photochemistry and Photobiology, Vol. 58, No. 1, 1993, p. 37-44.

Research output: Contribution to journalArticle

Lilge, L. ; Flotte, T. J. ; Kochevar, I. E. ; Jacques, Steven ; Hillenkamp, F. / PHOTOACTIVABLE FLUOROPHORES FOR THE MEASUREMENT OF FLUENCE IN TURBID MEDIA. In: Photochemistry and Photobiology. 1993 ; Vol. 58, No. 1. pp. 37-44.
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