Photoaccumulation in photosystem I does produce a phylloquinone (A1 •-) radical

Peter Heathcote, Pierre Moenne-Loccoz, Stephen E J Rigby, M. C W Evans

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Previous work has challenged the assignment of a photoaccumulated EPR signal to the phylloquinone electron acceptor in photosystem I, A1 •-. Biosynthetic deuteration of the phylloquinone in the cyanobacterium Anabaena variabilis has been shown to narrow this photoaccumulated signal, demonstrating that the signal arises from A1 •-. The ESP signal attributed to P700•+A1 •- is also narrowed by this deuteration, showing that the photoaccumulated EPR signal and the ESP signal are monitoring the same redox component. Confirmation that the photoaccumulated EPR signal comes from deuterated phylloquinone was obtained by exchanging the deuterated for protonated phylloquinone, which broadened the photoaccumulated EPR signal. † This work was supported by grants from the U.K. Biotechnology and Biological Sciences Research Council (BBSRC) and the University of London Intercollegiate Research Service (ULIRS).

Original languageEnglish (US)
Pages (from-to)6644-6650
Number of pages7
JournalBiochemistry
Volume35
Issue number21
StatePublished - May 28 1996
Externally publishedYes

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Vitamin K 1
Photosystem I Protein Complex
Paramagnetic resonance
Anabaena variabilis
Biological Science Disciplines
Organized Financing
Cyanobacteria
Biotechnology
Research
Oxidation-Reduction
Electrons
Monitoring

ASJC Scopus subject areas

  • Biochemistry

Cite this

Heathcote, P., Moenne-Loccoz, P., Rigby, S. E. J., & Evans, M. C. W. (1996). Photoaccumulation in photosystem I does produce a phylloquinone (A1 •-) radical. Biochemistry, 35(21), 6644-6650.

Photoaccumulation in photosystem I does produce a phylloquinone (A1 •-) radical. / Heathcote, Peter; Moenne-Loccoz, Pierre; Rigby, Stephen E J; Evans, M. C W.

In: Biochemistry, Vol. 35, No. 21, 28.05.1996, p. 6644-6650.

Research output: Contribution to journalArticle

Heathcote, P, Moenne-Loccoz, P, Rigby, SEJ & Evans, MCW 1996, 'Photoaccumulation in photosystem I does produce a phylloquinone (A1 •-) radical', Biochemistry, vol. 35, no. 21, pp. 6644-6650.
Heathcote, Peter ; Moenne-Loccoz, Pierre ; Rigby, Stephen E J ; Evans, M. C W. / Photoaccumulation in photosystem I does produce a phylloquinone (A1 •-) radical. In: Biochemistry. 1996 ; Vol. 35, No. 21. pp. 6644-6650.
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