Photo-releasable derivatives of inositol pyrophosphates

Sebastian Hauke, Tamara Bittner, Henning J. Jessen, Carsten Schultz

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The specific non-invasive control of intracellular signaling events requires advanced tools that enter cells by diffusion and are controllable by light. Here, we detail the synthesis and application of membrane-permeant caged inositol pyrophosphates with respect to cell entry and cell distribution. We recently published the synthesis of these tools as well as their effect on PH-domain localization in HeLa cells and oscillations of the intracellular calcium concentration in β-cells, which are known to drive insulin secretion. In this chapter, we discuss the possibilities and limitations when using cell-penetrating inositol pyrophosphates. We provide a detailed protocol for the application in live mouse β-cells and we discuss the image analysis needed for following effects on calcium signaling.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsDavid M. Chenoweth
PublisherAcademic Press Inc.
Pages53-73
Number of pages21
ISBN (Print)9780128211564
DOIs
StatePublished - 2020

Publication series

NameMethods in Enzymology
Volume641
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cell entry
  • Inositol phosphate
  • Photo-activation
  • Prodrugs
  • Signaling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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