Photo-releasable derivatives of inositol pyrophosphates

Sebastian Hauke, Tamara Bittner, Henning J. Jessen, Carsten Schultz

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The specific non-invasive control of intracellular signaling events requires advanced tools that enter cells by diffusion and are controllable by light. Here, we detail the synthesis and application of membrane-permeant caged inositol pyrophosphates with respect to cell entry and cell distribution. We recently published the synthesis of these tools as well as their effect on PH-domain localization in HeLa cells and oscillations of the intracellular calcium concentration in β-cells, which are known to drive insulin secretion. In this chapter, we discuss the possibilities and limitations when using cell-penetrating inositol pyrophosphates. We provide a detailed protocol for the application in live mouse β-cells and we discuss the image analysis needed for following effects on calcium signaling.

Original languageEnglish (US)
Title of host publicationChemical Tools for Imaging, Manipulating, and Tracking Biological Systems
Subtitle of host publicationDiverse Chemical, Optical and Bioorthogonal Methods
EditorsDavid M. Chenoweth
PublisherAcademic Press Inc.
Pages53-73
Number of pages21
ISBN (Print)9780128211564
DOIs
StatePublished - 2020

Publication series

NameMethods in Enzymology
Volume641
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cell entry
  • Inositol phosphate
  • Photo-activation
  • Prodrugs
  • Signaling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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