Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II

Ellen McGlade-McCulloh, Hideyuki Yamamoto, Soon Eng Tan, Debra A. Brickey, Thomas Soderling

    Research output: Contribution to journalArticle

    318 Citations (Scopus)

    Abstract

    THE major postsynaptic density (PSD) protein1,2 at glutaminergic synapses is calcium/calmodulin-dependent protein kinase II (CaM-K II), but its function in the PSD is not known. We have examined glutamate receptors (GluRs) as substrates for CaM-K II because (1) they are colocalized in the PSD3, (2) cloned GluRs4-7 contain consensus phosphorylation sites for protein kinases including CaM-K II, and (3) several GluRs are regulated by other protein kinases8-12. Regulation of GluRs, which are involved in excitatory synaptic transmission and in mechanisms of learning and memory13, by CaM-K II is of interest because of the postulated role of CaM-K II in synaptic plasticity14,15 and its known involvement in induction of long-term potentiation16. Furthermore, mice lacking the major neural isoform of CaM-K II exhibit deficits in models of learning and memory that require hippocampal input17,18. We report here that CaM-K II phosphorylates GluR in several in vitro systems, including the PSD, and that activated CaM-K II enhances kainate-induced ion current three- to fourfold in cultured hippocampal neurons. These results are consistent with a role for PSD CaM-K II in strengthening postsynaptic GluR responses in synaptic plasticity.

    Original languageEnglish (US)
    Pages (from-to)640-642
    Number of pages3
    JournalNature
    Volume362
    Issue number6421
    StatePublished - Apr 15 1993

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    Calcium-Calmodulin-Dependent Protein Kinase Type 2
    Calcium-Calmodulin-Dependent Protein Kinases
    Glutamate Receptors
    Phosphorylation
    Post-Synaptic Density
    Learning
    Neuronal Plasticity
    Kainic Acid
    Synaptic Transmission
    Synapses
    Protein Kinases
    Protein Isoforms
    Ions
    Neurons

    ASJC Scopus subject areas

    • General

    Cite this

    McGlade-McCulloh, E., Yamamoto, H., Tan, S. E., Brickey, D. A., & Soderling, T. (1993). Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II. Nature, 362(6421), 640-642.

    Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II. / McGlade-McCulloh, Ellen; Yamamoto, Hideyuki; Tan, Soon Eng; Brickey, Debra A.; Soderling, Thomas.

    In: Nature, Vol. 362, No. 6421, 15.04.1993, p. 640-642.

    Research output: Contribution to journalArticle

    McGlade-McCulloh, E, Yamamoto, H, Tan, SE, Brickey, DA & Soderling, T 1993, 'Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II', Nature, vol. 362, no. 6421, pp. 640-642.
    McGlade-McCulloh E, Yamamoto H, Tan SE, Brickey DA, Soderling T. Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II. Nature. 1993 Apr 15;362(6421):640-642.
    McGlade-McCulloh, Ellen ; Yamamoto, Hideyuki ; Tan, Soon Eng ; Brickey, Debra A. ; Soderling, Thomas. / Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II. In: Nature. 1993 ; Vol. 362, No. 6421. pp. 640-642.
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