Phosphorylation and inhibition of olfactory adenylyl cyclase by CaM kinase II in neurons

A mechanism for attenuation of olfactory signals

Jia Wei, Allan Z. Zhao, Guy C K Chan, Lauren P. Baker, Soren Impey, Joseph A. Beavo, Daniel R. Storm

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Acute desensitization of olfactory signaling is a critical property of the olfactory system that allows animals to detect and respond to odorants. Correspondingly, an important feature of odorant-stimulated cAMP increases is their transient nature, a phenomenon that may be attributable to the unique regulatory properties of the olfactory adenylyl cyclase (AC3). AC3 is stimulated by receptor activation and inhibited by Ca2+ through Ca2+/calmodulin kinase II (CaMKII) phosphorylation at Ser-1076. Since odorant-stimulated cAMP increases are accompanied by elevated intracellular Ca2+, CaMKII inhibition of AC3 may contribute to termination of olfactory signaling. To test this hypothesis, we generated a polyclonal antibody specific for AC3 phosphorylated at Ser-1076. A brief exposure of mouse olfactory cilia or primary olfactory neurons to odorants stimulated phosphorylation of AC3 at Ser-1076. This phosphorylation was blocked by inhibitors of CaMKII, which also ablated cAMP decreases associated with odorant-stimulated cAMP transients. These data define a novel mechanism for termination of olfactory signaling that may be important in olfactory responses.

Original languageEnglish (US)
Pages (from-to)495-504
Number of pages10
JournalNeuron
Volume21
Issue number3
DOIs
StatePublished - Sep 1998
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Adenylyl Cyclases
Calcium-Calmodulin-Dependent Protein Kinases
Phosphorylation
Neurons
Cilia
Odorants
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Phosphorylation and inhibition of olfactory adenylyl cyclase by CaM kinase II in neurons : A mechanism for attenuation of olfactory signals. / Wei, Jia; Zhao, Allan Z.; Chan, Guy C K; Baker, Lauren P.; Impey, Soren; Beavo, Joseph A.; Storm, Daniel R.

In: Neuron, Vol. 21, No. 3, 09.1998, p. 495-504.

Research output: Contribution to journalArticle

Wei, Jia ; Zhao, Allan Z. ; Chan, Guy C K ; Baker, Lauren P. ; Impey, Soren ; Beavo, Joseph A. ; Storm, Daniel R. / Phosphorylation and inhibition of olfactory adenylyl cyclase by CaM kinase II in neurons : A mechanism for attenuation of olfactory signals. In: Neuron. 1998 ; Vol. 21, No. 3. pp. 495-504.
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