Phosphorylated CREB binds specifically to the nuclear protein CBP

John C. Chrivia, Roland P S Kwok, Ned Lamb, Masatoshi Hagiwara, Marc R. Montminy, Richard Goodman

Research output: Contribution to journalArticle

1660 Citations (Scopus)

Abstract

CYCLIC AMP-regulated gene expression frequently involves a DNA element known as the cAMP-regulated enhancer (CRE)1-4. Many transcription factors bind to this element, including the protein CREB5,6, which is activated as a result of phosphorylation by protein kinase A7. This modification stimulates interaction with one or more of the general transcription factors or, alternatively, allows recruitment of a co-activator. Here we report that CREB phosphorylated by protein kinase A binds specifically to a nuclear protein of Mr 265K which we term CBP (for CREB-binding protein). Fusion of a heterologous DNA-binding domain to the amino terminus of CBP enables the chimaeric protein to function as a protein kinase A-regulated transcriptional activator. We propose that CBP may participate in cAMP-regulated gene expression by interacting with the activated phosphorylated form of CREB.

Original languageEnglish (US)
JournalNature
Volume365
Issue number6449
StatePublished - Oct 28 1993

Fingerprint

CREB-Binding Protein
Nuclear Proteins
Protein Binding
Cyclic AMP-Dependent Protein Kinases
General Transcription Factors
Gene Expression
Cyclic AMP Response Element-Binding Protein
DNA
Adenosine Monophosphate
Protein Kinases
Proteins
Transcription Factors
Phosphorylation

ASJC Scopus subject areas

  • General

Cite this

Chrivia, J. C., Kwok, R. P. S., Lamb, N., Hagiwara, M., Montminy, M. R., & Goodman, R. (1993). Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature, 365(6449).

Phosphorylated CREB binds specifically to the nuclear protein CBP. / Chrivia, John C.; Kwok, Roland P S; Lamb, Ned; Hagiwara, Masatoshi; Montminy, Marc R.; Goodman, Richard.

In: Nature, Vol. 365, No. 6449, 28.10.1993.

Research output: Contribution to journalArticle

Chrivia, JC, Kwok, RPS, Lamb, N, Hagiwara, M, Montminy, MR & Goodman, R 1993, 'Phosphorylated CREB binds specifically to the nuclear protein CBP', Nature, vol. 365, no. 6449.
Chrivia JC, Kwok RPS, Lamb N, Hagiwara M, Montminy MR, Goodman R. Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature. 1993 Oct 28;365(6449).
Chrivia, John C. ; Kwok, Roland P S ; Lamb, Ned ; Hagiwara, Masatoshi ; Montminy, Marc R. ; Goodman, Richard. / Phosphorylated CREB binds specifically to the nuclear protein CBP. In: Nature. 1993 ; Vol. 365, No. 6449.
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