(Phospho)Proteomic dataset of ischemia- and ultrasound- stimulated mouse cardiac endothelial cells in vitro

Uchenna Emechebe, David Giraud, Azzdine Ammi, Kristin L. Scott, Jon M. Jacobs, Jason E. McDermott, Igor V. Dykan, Nabil J. Alkayed, Anthony P. Barnes, Sanjiv Kaul, Catherine M. Davis

Research output: Contribution to journalArticlepeer-review

Abstract

Cardiac endothelial cells respond to both ischemia and therapeutic ultrasound; the proteomic changes underlying these responses are unknown. This data article provides raw and processed data resulting from our global, unbiased phosphoproteomics investigation conducted on primary mouse cardiac endothelial cells exposed to ischemia (2-hour oxygen glucose deprivation) and ultrasound (250 kHz, 1.2 MPa) in vitro [1]. Proteins were extracted from cell lysates and enriched phosphopeptides were analyzed with a high mass accuracy liquid chromatrography (LC) - tandem mass spectrometry (MS/MS) proteomic platform, yielding multiple alterations in both total protein levels and phosphorylation events in response to ischemic injury and ultrasound. This dataset can be used as a reference for future studies on the cardiac endothelial response to ischemia and the mechanistic underpinnings of the cellular response to ultrasound, with the potential to yield clinically relevant therapeutic targets.

Original languageEnglish (US)
Article number107343
JournalData in Brief
Volume38
DOIs
StatePublished - Oct 2021

Keywords

  • Endothelial cell
  • Heart
  • Ischemia
  • Phosphoproteomics
  • Proteomics
  • Ultrasound

ASJC Scopus subject areas

  • General

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