Phospholipid methylation in myogenic cells

Thomas K. Koch, Adrienne S. Gordon, Ivan Diamond

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The biosynthesis of phosphatidylcholine from successive N-methylation of phosphatidylethanolamine has been implicated as a major mechanism for the transduction of several receptor-mediated signals including β -adrenergic coupling to adenylate cyclase. In this report we demonstrate L-isoproterenol stimulation of adenylate cyclase activity in two myogenic cell lines, L8 and BC3H-1. Using a sensitive high performance liquid chromatography method for qualitative and quantitative determination of phospholipids we found an active membrane phospholipid methylation pathway in these cells. Despite β -adrenergic simulation of adenylate cyclase no alteration in the transmethylation pathway could be demonstrated.

Original languageEnglish (US)
Pages (from-to)339-347
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume114
Issue number1
DOIs
StatePublished - Jul 18 1983

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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