Phosphatidylserine-positive particles in the apical domain of sensory hair cells

Shi Xiao-rui, Alfred Nuttall

Research output: Contribution to journalArticle

Abstract

Apical membrane recycling has been proposed to be important for normal hair cell function. The current study reports an in vitro work that demonstrates the presence of phosphatidylserine (PS) and PS-positive vesicles labeled by Annexin V in the apical portion of hair cells. The following characteristics of the PS-positive vesicles were noticed using scanning confocal fluorescence microscopy:(1) variable sizes around 200 nm; (2) variable distribution patterns (either uniformly along individual stereocilia in the hair bundle or irregular) in the stereocilia from cell to cell; (3) variable sizes and numbers at locations along the border of the cuticular plate (CP), with a large number of them located at the vestigal kinocilial location; (4) motility with some of the vesicles during the observation period; (5) increase in PS labeling and the number of PS-positive vesicles after loud sound stimulation; and (6) decreased PS labeling and PS-positive vesicle numbers following treatment with LY-294002, a PI3 -kinase inhibitor. These results suggest that the presence of PS-positive vesicles at the apical area of hair cells may be indicative of vesicle shedding or transportation of a protein or rafts.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalJournal of Otology
Volume1
Issue number2
DOIs
StatePublished - Dec 1 2006

Fingerprint

Phosphatidylserines
Stereocilia
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Annexin A5
Phosphatidylinositol 3-Kinases
Fluorescence Microscopy
Confocal Microscopy
Observation
Membranes

Keywords

  • Annexin V
  • Hair cell
  • PS
  • PS-positive particles

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Phosphatidylserine-positive particles in the apical domain of sensory hair cells. / Xiao-rui, Shi; Nuttall, Alfred.

In: Journal of Otology, Vol. 1, No. 2, 01.12.2006, p. 76-82.

Research output: Contribution to journalArticle

@article{5722c30e97b5478dbd7b9c56cd31c7a5,
title = "Phosphatidylserine-positive particles in the apical domain of sensory hair cells",
abstract = "Apical membrane recycling has been proposed to be important for normal hair cell function. The current study reports an in vitro work that demonstrates the presence of phosphatidylserine (PS) and PS-positive vesicles labeled by Annexin V in the apical portion of hair cells. The following characteristics of the PS-positive vesicles were noticed using scanning confocal fluorescence microscopy:(1) variable sizes around 200 nm; (2) variable distribution patterns (either uniformly along individual stereocilia in the hair bundle or irregular) in the stereocilia from cell to cell; (3) variable sizes and numbers at locations along the border of the cuticular plate (CP), with a large number of them located at the vestigal kinocilial location; (4) motility with some of the vesicles during the observation period; (5) increase in PS labeling and the number of PS-positive vesicles after loud sound stimulation; and (6) decreased PS labeling and PS-positive vesicle numbers following treatment with LY-294002, a PI3 -kinase inhibitor. These results suggest that the presence of PS-positive vesicles at the apical area of hair cells may be indicative of vesicle shedding or transportation of a protein or rafts.",
keywords = "Annexin V, Hair cell, PS, PS-positive particles",
author = "Shi Xiao-rui and Alfred Nuttall",
year = "2006",
month = "12",
day = "1",
doi = "10.1016/S1672-2930(06)50017-0",
language = "English (US)",
volume = "1",
pages = "76--82",
journal = "Journal of Otology",
issn = "1672-2930",
publisher = "PLA General Hospital Department of Otolaryngology Head and Neck Surgery",
number = "2",

}

TY - JOUR

T1 - Phosphatidylserine-positive particles in the apical domain of sensory hair cells

AU - Xiao-rui, Shi

AU - Nuttall, Alfred

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Apical membrane recycling has been proposed to be important for normal hair cell function. The current study reports an in vitro work that demonstrates the presence of phosphatidylserine (PS) and PS-positive vesicles labeled by Annexin V in the apical portion of hair cells. The following characteristics of the PS-positive vesicles were noticed using scanning confocal fluorescence microscopy:(1) variable sizes around 200 nm; (2) variable distribution patterns (either uniformly along individual stereocilia in the hair bundle or irregular) in the stereocilia from cell to cell; (3) variable sizes and numbers at locations along the border of the cuticular plate (CP), with a large number of them located at the vestigal kinocilial location; (4) motility with some of the vesicles during the observation period; (5) increase in PS labeling and the number of PS-positive vesicles after loud sound stimulation; and (6) decreased PS labeling and PS-positive vesicle numbers following treatment with LY-294002, a PI3 -kinase inhibitor. These results suggest that the presence of PS-positive vesicles at the apical area of hair cells may be indicative of vesicle shedding or transportation of a protein or rafts.

AB - Apical membrane recycling has been proposed to be important for normal hair cell function. The current study reports an in vitro work that demonstrates the presence of phosphatidylserine (PS) and PS-positive vesicles labeled by Annexin V in the apical portion of hair cells. The following characteristics of the PS-positive vesicles were noticed using scanning confocal fluorescence microscopy:(1) variable sizes around 200 nm; (2) variable distribution patterns (either uniformly along individual stereocilia in the hair bundle or irregular) in the stereocilia from cell to cell; (3) variable sizes and numbers at locations along the border of the cuticular plate (CP), with a large number of them located at the vestigal kinocilial location; (4) motility with some of the vesicles during the observation period; (5) increase in PS labeling and the number of PS-positive vesicles after loud sound stimulation; and (6) decreased PS labeling and PS-positive vesicle numbers following treatment with LY-294002, a PI3 -kinase inhibitor. These results suggest that the presence of PS-positive vesicles at the apical area of hair cells may be indicative of vesicle shedding or transportation of a protein or rafts.

KW - Annexin V

KW - Hair cell

KW - PS

KW - PS-positive particles

UR - http://www.scopus.com/inward/record.url?scp=85071939370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071939370&partnerID=8YFLogxK

U2 - 10.1016/S1672-2930(06)50017-0

DO - 10.1016/S1672-2930(06)50017-0

M3 - Article

AN - SCOPUS:85071939370

VL - 1

SP - 76

EP - 82

JO - Journal of Otology

JF - Journal of Otology

SN - 1672-2930

IS - 2

ER -