Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana

Dayana Rodriguez-Contreras, Xiuhong Feng, Kristie M. Keeney, H. G Archie Bouwer, Scott Landfear

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Glucose is a major source of energy and carbon in promastigotes of Leishmania mexicana, and its uptake is mediated by three glucose transporters whose genes are encoded within a single cluster. A null mutant in which the glucose transporter gene cluster was deleted by homologous gene replacement was generated previously and shown to grow more slowly than wild type promastigotes but not to be viable as amastigotes in primary tissue culture macrophages or in axenic culture. Further phenotypic characterization demonstrates that the null mutant is unable to import glucose, mannose, fructose, or galactose and that each of the three glucose transporter isoforms, LmGT1, LmGT2, and LmGT3, is capable of transporting each of these hexoses. Complementation of the null mutant with each isoform is able to restore growth in each of the four hexoses to wild type levels. Null mutant promastigotes are reduced in size to about 2/3 the volume of wild type parasites. In addition, the null mutants are significantly more sensitive to oxidative stress than their wild type counterparts. These results underscore the importance of glucose transporters in the parasite life cycle and suggest reasons for their non-viability in the disease-causing amastigote stage.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalMolecular and Biochemical Parasitology
Volume153
Issue number1
DOIs
StatePublished - May 2007

Fingerprint

Leishmania mexicana
Facilitative Glucose Transport Proteins
Hexoses
Protein Isoforms
Parasites
Axenic Culture
Glucose
Mannose
Multigene Family
Fructose
Life Cycle Stages
Galactose
Genes
Oxidative Stress
Carbon
Macrophages
Growth
Leishmania glucose transporter

Keywords

  • Glucose transporters
  • Hexose transport
  • Leishmania mexicana
  • Null mutant
  • Oxidative stress

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. / Rodriguez-Contreras, Dayana; Feng, Xiuhong; Keeney, Kristie M.; Bouwer, H. G Archie; Landfear, Scott.

In: Molecular and Biochemical Parasitology, Vol. 153, No. 1, 05.2007, p. 9-18.

Research output: Contribution to journalArticle

Rodriguez-Contreras, Dayana ; Feng, Xiuhong ; Keeney, Kristie M. ; Bouwer, H. G Archie ; Landfear, Scott. / Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. In: Molecular and Biochemical Parasitology. 2007 ; Vol. 153, No. 1. pp. 9-18.
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