Pharmacological characterization of type II glucocorticoid binding sites in AtT20 pituitary cell culture

M. N. Gannon, R. L. Spencer, James Lundblad, B. S. McEwen, J. L. Roberts

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Recent evidence indicates that at least two functional glucocorticoid receptors (Type I and Type II) are present in many tissues. It has also become increasingly recognized that, as in other systems, stimulus-response relationships for steroid hormones are often nonlinear. Thus, precise pharmacological parameters are required to establish a functional relationship(s) between binding site and response characteristics. We therefore pharmacologically characterized a glucocorticoid binding site present in AtT20 mouse pituitary cells, a cell line extensively used in studying Type II glucocorticoid receptor function. By several different criteria, glucocorticoids were shown to bind to a single class of binding sites, which, in comparison to available literature, correspond to classical Type II glucocorticoid receptors. No evidence for Type I adrenal steroid binding sites was observed, under the experimental conditions used. Unambiguous Kb values for both glucocorticoid agonists and antagonists were therefore calculated. These parameters should prove of use in elucidating the relationships between glucocorticoid receptor activation and different responses in both AtT20 cells and other glucocorticoid responsive tissues.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalJournal of Steroid Biochemistry
Volume36
Issue number1-2
DOIs
StatePublished - Jun 8 1990
Externally publishedYes

Fingerprint

Glucocorticoid Receptors
Cell culture
Glucocorticoids
Cell Culture Techniques
Binding Sites
Pharmacology
Steroid hormones
Steroids
Tissue
Chemical activation
Cells
Hormones
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Pharmacological characterization of type II glucocorticoid binding sites in AtT20 pituitary cell culture. / Gannon, M. N.; Spencer, R. L.; Lundblad, James; McEwen, B. S.; Roberts, J. L.

In: Journal of Steroid Biochemistry, Vol. 36, No. 1-2, 08.06.1990, p. 83-88.

Research output: Contribution to journalArticle

Gannon, M. N. ; Spencer, R. L. ; Lundblad, James ; McEwen, B. S. ; Roberts, J. L. / Pharmacological characterization of type II glucocorticoid binding sites in AtT20 pituitary cell culture. In: Journal of Steroid Biochemistry. 1990 ; Vol. 36, No. 1-2. pp. 83-88.
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