Phagocytosis of individual CD4+ T cells by HIV-induced T cell syncytia

S. Murphy, Andrew Sylwester, R. C. Kennedy, D. R. Soll

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Transmission electron microscopic analysis of HIV-induced syncytia of the CD4+ SupT1 cell line has revealed profiles of whole T cells in the syncytium cytoplasm. Serial sections demonstrate that these T cells are completely enveloped by a second membrane in the syncytium cytoplasm and represent phagosomes. Pycnosis of engulfed T cell nuclei, vacuolation of the cytoplasm of engulfed T cells, and the association of engulfed T cells with dense vesicular clusters in the syncytium cytoplasm support the conclusion that they represent phagosomes. In addition, transmission electron micrographs of the syncytium surface reveal giant pseudopodial extensions wrapping around T cells, in a fashion similar to bacterial and yeast phagocytosis by professional phagocytes. These results suggest that phagocytosis is a characteristic acquired during HIV-induced syncytium formation, and that it may represent an avenue of T cell death in addition to fusion in HIV-infected SupT1 cell cultures.

Original languageEnglish (US)
Pages (from-to)433-442
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume11
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Giant Cells
Phagocytosis
HIV
T-Lymphocytes
Cytoplasm
Phagosomes
Electrons
Phagocytes
Cell Nucleus
Cell Death
Cell Culture Techniques
Yeasts
Cell Line
Membranes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Phagocytosis of individual CD4+ T cells by HIV-induced T cell syncytia. / Murphy, S.; Sylwester, Andrew; Kennedy, R. C.; Soll, D. R.

In: AIDS Research and Human Retroviruses, Vol. 11, No. 4, 1995, p. 433-442.

Research output: Contribution to journalArticle

Murphy, S. ; Sylwester, Andrew ; Kennedy, R. C. ; Soll, D. R. / Phagocytosis of individual CD4+ T cells by HIV-induced T cell syncytia. In: AIDS Research and Human Retroviruses. 1995 ; Vol. 11, No. 4. pp. 433-442.
@article{c61af199467f42d7af4328cd9c596c03,
title = "Phagocytosis of individual CD4+ T cells by HIV-induced T cell syncytia",
abstract = "Transmission electron microscopic analysis of HIV-induced syncytia of the CD4+ SupT1 cell line has revealed profiles of whole T cells in the syncytium cytoplasm. Serial sections demonstrate that these T cells are completely enveloped by a second membrane in the syncytium cytoplasm and represent phagosomes. Pycnosis of engulfed T cell nuclei, vacuolation of the cytoplasm of engulfed T cells, and the association of engulfed T cells with dense vesicular clusters in the syncytium cytoplasm support the conclusion that they represent phagosomes. In addition, transmission electron micrographs of the syncytium surface reveal giant pseudopodial extensions wrapping around T cells, in a fashion similar to bacterial and yeast phagocytosis by professional phagocytes. These results suggest that phagocytosis is a characteristic acquired during HIV-induced syncytium formation, and that it may represent an avenue of T cell death in addition to fusion in HIV-infected SupT1 cell cultures.",
author = "S. Murphy and Andrew Sylwester and Kennedy, {R. C.} and Soll, {D. R.}",
year = "1995",
language = "English (US)",
volume = "11",
pages = "433--442",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Phagocytosis of individual CD4+ T cells by HIV-induced T cell syncytia

AU - Murphy, S.

AU - Sylwester, Andrew

AU - Kennedy, R. C.

AU - Soll, D. R.

PY - 1995

Y1 - 1995

N2 - Transmission electron microscopic analysis of HIV-induced syncytia of the CD4+ SupT1 cell line has revealed profiles of whole T cells in the syncytium cytoplasm. Serial sections demonstrate that these T cells are completely enveloped by a second membrane in the syncytium cytoplasm and represent phagosomes. Pycnosis of engulfed T cell nuclei, vacuolation of the cytoplasm of engulfed T cells, and the association of engulfed T cells with dense vesicular clusters in the syncytium cytoplasm support the conclusion that they represent phagosomes. In addition, transmission electron micrographs of the syncytium surface reveal giant pseudopodial extensions wrapping around T cells, in a fashion similar to bacterial and yeast phagocytosis by professional phagocytes. These results suggest that phagocytosis is a characteristic acquired during HIV-induced syncytium formation, and that it may represent an avenue of T cell death in addition to fusion in HIV-infected SupT1 cell cultures.

AB - Transmission electron microscopic analysis of HIV-induced syncytia of the CD4+ SupT1 cell line has revealed profiles of whole T cells in the syncytium cytoplasm. Serial sections demonstrate that these T cells are completely enveloped by a second membrane in the syncytium cytoplasm and represent phagosomes. Pycnosis of engulfed T cell nuclei, vacuolation of the cytoplasm of engulfed T cells, and the association of engulfed T cells with dense vesicular clusters in the syncytium cytoplasm support the conclusion that they represent phagosomes. In addition, transmission electron micrographs of the syncytium surface reveal giant pseudopodial extensions wrapping around T cells, in a fashion similar to bacterial and yeast phagocytosis by professional phagocytes. These results suggest that phagocytosis is a characteristic acquired during HIV-induced syncytium formation, and that it may represent an avenue of T cell death in addition to fusion in HIV-infected SupT1 cell cultures.

UR - http://www.scopus.com/inward/record.url?scp=0029029255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029029255&partnerID=8YFLogxK

M3 - Article

C2 - 7632459

AN - SCOPUS:0029029255

VL - 11

SP - 433

EP - 442

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 4

ER -