Phage display of a catalytic antibody to optimize affinity for transition-state analog binding

Manuel Baca, Thomas (Tom) Scanlan, Robert C. Stephenson, James A. Wells

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Catalytic antibodies have shown great promise for catalyzing a tremendously diverse set of natural and unnatural chemical transformations. However, few catalytic antibodies have efficiencies that approach those of natural enzymes. In principle, random mutagenesis procedures such as phage display could be used to improve the catalytic activities of existing antibodies; however, these studies have been hampered by difficulties in the recombinant expression of antibodies. Here, we have grafted the antigen binding loops from a murine-derived catalytic antibody, 17E8, onto a human antibody framework in an effort to overcome difficulties associated with recombinant expression and phage display of this antibody. 'Humanized' 17E8 retained similar catalytic and hapten binding properties as the murine antibody while levels of functional Fab displayed on phage were 200-fold higher than for a murine variable region/human constant region chimeric Fab. This construct was used to prepare combinatorial libraries. Affinity panning of these resulted in the selection of variants with 2- to 8-fold improvements in binding affinity for a phosphonate transition-state analog. Surprisingly, none of the affinity-matured variants was more catalytically active than the parent antibody and some were significantly less active. By contrast, a weaker binding variant was identified with 2-fold greater catalytic activity and incorporation of a single substitution (Tyr-100(aH) → Asn) from this variant into the parent antibody led to a 5-fold increase in catalytic efficiency. Thus, phage display methods can be readily used to optimize binding of catalytic antibodies to transition-state analogs, and when used in conjunction with limited screening for catalysis can identify variants with higher catalytic efficiencies.

Original languageEnglish (US)
Pages (from-to)10063-10068
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number19
DOIs
StatePublished - Sep 16 1997
Externally publishedYes

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Catalytic Antibodies
Bacteriophages
Antibodies
Organophosphonates
Haptens
Catalysis
Mutagenesis
Antigens

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Phage display of a catalytic antibody to optimize affinity for transition-state analog binding. / Baca, Manuel; Scanlan, Thomas (Tom); Stephenson, Robert C.; Wells, James A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 19, 16.09.1997, p. 10063-10068.

Research output: Contribution to journalArticle

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