Phage 8-9 defines a cluster of site polymorphisms on chromosome 16q22-q24 [HGM9 no. D16S20]

C. Maslen; R. Ellen Magenis; Robert Sheehy; M. Litt

doi:10.1093/nar/16.16.8195

Phage 8-9 defines a cluster of site polymorphisms on chromosome 16q22-q24 [HGM9 no. D16S20]

C. Maslen, R. Ellen Magenis, Robert Sheehy, M. Litt

Research output: Contribution to journal › Article › peer-review

Original language	English (US)
Pages (from-to)	8195
Number of pages	1
Journal	Nucleic acids research
Volume	16
Issue number	16
DOIs	https://doi.org/10.1093/nar/16.16.8195
State	Published - Aug 25 1988

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Genetics

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@article{d16959e000374f268383ea1253b80d08,

title = "Phage 8-9 defines a cluster of site polymorphisms on chromosome 16q22-q24 [HGM9 no. D16S20]",

author = "C. Maslen and Magenis, {R. Ellen} and Robert Sheehy and M. Litt",

note = "Funding Information: from a genomic library of a mouse x human somatic cell hybrid (CF-52) containing an 11q-16q translocation as the only human chromosome. A 17 kb partial Sau 3A fragment was cloned in the BamHI site of EMBL3. POLYMORPHISMS: Sac I identifies constant bands at 4.1, 2.3 and 1.3 kb and two 2-allele RFLPs with A1-10, A2=7.4 +2.6 kb and B1=2.9, B2=1.9+1.0 kb. Bgl II identifies a constant band of 8 kb and a 3-allale RFLP with C1>20, C2=14 and C3=8.5 +5.2 kb. Pvu II identifies 8 constant bands <3.5 kb and a 2-allele RFLP with D1 - 6.5, D2=5.8+0.7 kb. FREQUENCIES: Studied at least 34 European Caucasians. A1=.43, A2=.57; B1=.26,B2=.74; C1 =.03, C2=.71, C3=.29; D1-.32, D2=.68. CHROMOSOMAL LOCALIZATION: 16q22-q24, by in situ hybridization. Localized approx. 7 cM distal to CTRB on CEPH linkage map of chromosome 16. MENDELIAN INHERITANCE: Codominant inheritance has been shown at each of the four loci in at least 2 informative families with a total of at least 13 children. PROBE AVAILABILITY: Will be available from ATCC. OTHER COMMENTS: The probe must be prehybridized with excess human DNA to compete out hybridization of repeated sequences. Of 36 unrelated individuals typed at loci A, B and D, 31 (86%) were heterozygous at one or more of the 3 loci. D16S20 is also polymorphic with Pst I, Bgl I, Taq I, Msp I, BstN I, Asp 700, Bel I and Hae III, but not with EcoR I, Hind III, BamH I, Rsa I, Xba I, Apa I or Dra I. For 428 bp screened, the average heterozygosity per bp was .0072. REFERENCES: 1.)CL Maslen, C Jones, T Glaser, RE Magenis, R Sheehy, J Kellogg and M Litt(1988)Genomics 2:66-75. 2.)GM Lathrop, M Leppert, Y Nakamura, P O'Connell, M Litt, C Maslen, P Cartwright, J-M Lalouel and R White. (1988) Cytogenet Cell Genet, in press. ACKNOWLEDGEMENTS: Supported by grants from NIH (RO1-GM32500) and the Retinitis Pigmentosum Foundation Fighting Blindness.",

year = "1988",

month = aug,

day = "25",

doi = "10.1093/nar/16.16.8195",

language = "English (US)",

volume = "16",

pages = "8195",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "16",

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TY - JOUR

T1 - Phage 8-9 defines a cluster of site polymorphisms on chromosome 16q22-q24 [HGM9 no. D16S20]

AU - Maslen, C.

AU - Magenis, R. Ellen

AU - Sheehy, Robert

AU - Litt, M.

N1 - Funding Information: from a genomic library of a mouse x human somatic cell hybrid (CF-52) containing an 11q-16q translocation as the only human chromosome. A 17 kb partial Sau 3A fragment was cloned in the BamHI site of EMBL3. POLYMORPHISMS: Sac I identifies constant bands at 4.1, 2.3 and 1.3 kb and two 2-allele RFLPs with A1-10, A2=7.4 +2.6 kb and B1=2.9, B2=1.9+1.0 kb. Bgl II identifies a constant band of 8 kb and a 3-allale RFLP with C1>20, C2=14 and C3=8.5 +5.2 kb. Pvu II identifies 8 constant bands <3.5 kb and a 2-allele RFLP with D1 - 6.5, D2=5.8+0.7 kb. FREQUENCIES: Studied at least 34 European Caucasians. A1=.43, A2=.57; B1=.26,B2=.74; C1 =.03, C2=.71, C3=.29; D1-.32, D2=.68. CHROMOSOMAL LOCALIZATION: 16q22-q24, by in situ hybridization. Localized approx. 7 cM distal to CTRB on CEPH linkage map of chromosome 16. MENDELIAN INHERITANCE: Codominant inheritance has been shown at each of the four loci in at least 2 informative families with a total of at least 13 children. PROBE AVAILABILITY: Will be available from ATCC. OTHER COMMENTS: The probe must be prehybridized with excess human DNA to compete out hybridization of repeated sequences. Of 36 unrelated individuals typed at loci A, B and D, 31 (86%) were heterozygous at one or more of the 3 loci. D16S20 is also polymorphic with Pst I, Bgl I, Taq I, Msp I, BstN I, Asp 700, Bel I and Hae III, but not with EcoR I, Hind III, BamH I, Rsa I, Xba I, Apa I or Dra I. For 428 bp screened, the average heterozygosity per bp was .0072. REFERENCES: 1.)CL Maslen, C Jones, T Glaser, RE Magenis, R Sheehy, J Kellogg and M Litt(1988)Genomics 2:66-75. 2.)GM Lathrop, M Leppert, Y Nakamura, P O'Connell, M Litt, C Maslen, P Cartwright, J-M Lalouel and R White. (1988) Cytogenet Cell Genet, in press. ACKNOWLEDGEMENTS: Supported by grants from NIH (RO1-GM32500) and the Retinitis Pigmentosum Foundation Fighting Blindness.

PY - 1988/8/25

Y1 - 1988/8/25

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JO - Nucleic acids research

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ER -

Phage 8-9 defines a cluster of site polymorphisms on chromosome 16q22-q24 [HGM9 no. D16S20]

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