PHA activation of encephalitogenic T cells

In vitro line selection overcomes splenic suppression

R. E. Jones, Halina Offner, G. Dietsch, D. Hinrichs, Arthur Vandenbark

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study we compared myelin basic protein (MBP) and phytohemagglutinin (PHA) for their ability to induce proliferation and experimental autoimmune encephalomyelitis (EAE) transfer activity in mixed cell cultures obtained from spleen and lymph nodes versus highly selected MBP-specific T cell lines and clones. Established MBP-specific cells derived initially from immune lymph nodes attained both proliferative and EAE-transfer activities after in vitro activation with either MBP or PHA. In contrast, PHA was unable to induce immune spleen cells to transfer EAE, in spite of its potent mitogenic activity. On the basis of these results, we evaluated the in vitro proliferation and differentiation responses of MBP-specific T cells during the line selection process using cells derived from both immune lymph node and immune spleen. During the initial selection process with MBP, proliferation of MBP-specific T cell precursors from immunized spleen populations was reduced relative to lymph node cells. After antigen-dependent selection the encephalitogenic cells from either organ exhibited identical in vitro response characteristics. Freshly isolated immune spleen cells were potent suppressors of MBP-specific T cell proliferation suggesting that the in vitro differences between the two organs was due to splenic suppression of the encephalitogenic cells.

Original languageEnglish (US)
Pages (from-to)60-69
Number of pages10
JournalCellular Immunology
Volume123
Issue number1
DOIs
StatePublished - Oct 1 1989

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Myelin Basic Protein
Phytohemagglutinins
T-Lymphocytes
Spleen
Autoimmune Experimental Encephalomyelitis
Lymph Nodes
T-Lymphoid Precursor Cells
Cell Line
In Vitro Techniques
Clone Cells
Cell Culture Techniques
Cell Proliferation
Antigens

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

PHA activation of encephalitogenic T cells : In vitro line selection overcomes splenic suppression. / Jones, R. E.; Offner, Halina; Dietsch, G.; Hinrichs, D.; Vandenbark, Arthur.

In: Cellular Immunology, Vol. 123, No. 1, 01.10.1989, p. 60-69.

Research output: Contribution to journalArticle

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