PET imaging of dopamine D2 receptors with [18F]fluoroclebopride in monkeys: Effects of isoflurane- and ketamine-induced anesthesia

Michael A. Nader, Kathleen (Kathy) Grant, H. Donald Gage, Richard L. Ehrenkaufer, Jay R. Kaplan, Robert H. MacH

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The purpose of the present study was to determine whether positron emission tomography (PET) studies in monkeys with the dopamine (DA) D2 receptor ligand [18F]fluoroclebopride (FCP) would be significantly influenced by anesthetic induction with isoflurane (~5.0%) compared to induction with 10 mg/kg ketamine. Five experimentally-naive adult male cynomolgus monkeys (Macaca fascicularis) were trained to sit calmly in a primate restraint chair. Before the first PET scan, each monkey was anesthetized, by mask, with isoflurane. After complete sedation, the monkey was intubated and anesthesia was maintained throughout the PET study by isoflurane (~1.5%). At least 1 month later, a second PET study was conducted in which anesthesia was induced with ketamine and maintained by isoflurane (~1.5%). Irrespective of induction anesthetic, there was a high uptake of [18F]FCP and a linear rate of washout from the basal ganglia for all monkeys. There were also no differences in time to peak uptake (~25 min), in clearance half-life (t(1/2)= 140-164 min) or in D2 binding (distribution volume ratios of 2.48 vs. 2.50). These results indicate that induction anesthetic did not differentially affect D2 binding of [18F]FCP in monkeys. Furthermore, the low variability between studies indicates that [18F]FCP is an excellent ligand for longitudinal studies of D2 receptors in nonhuman primates. Copyright (C) 1999 American College of Neuropsychopharmacology.

Original languageEnglish (US)
Pages (from-to)589-596
Number of pages8
JournalNeuropsychopharmacology
Volume21
Issue number4
DOIs
StatePublished - Oct 1999
Externally publishedYes

Fingerprint

Dopamine D2 Receptors
Isoflurane
Ketamine
Positron-Emission Tomography
Haplorhini
Anesthesia
Anesthetics
Macaca fascicularis
Primates
Ligands
Masks
Basal Ganglia
Longitudinal Studies
Half-Life
fluoroclebopride

Keywords

  • Dopamine D receptors
  • Fluoroclebopride
  • Isoflurane
  • Ketamine
  • Monkey
  • Positron emission tomography

ASJC Scopus subject areas

  • Pharmacology

Cite this

PET imaging of dopamine D2 receptors with [18F]fluoroclebopride in monkeys : Effects of isoflurane- and ketamine-induced anesthesia. / Nader, Michael A.; Grant, Kathleen (Kathy); Gage, H. Donald; Ehrenkaufer, Richard L.; Kaplan, Jay R.; MacH, Robert H.

In: Neuropsychopharmacology, Vol. 21, No. 4, 10.1999, p. 589-596.

Research output: Contribution to journalArticle

Nader, Michael A. ; Grant, Kathleen (Kathy) ; Gage, H. Donald ; Ehrenkaufer, Richard L. ; Kaplan, Jay R. ; MacH, Robert H. / PET imaging of dopamine D2 receptors with [18F]fluoroclebopride in monkeys : Effects of isoflurane- and ketamine-induced anesthesia. In: Neuropsychopharmacology. 1999 ; Vol. 21, No. 4. pp. 589-596.
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