Persistent Subepidermal Blistering in Split-Thickness Skin Graft Sites

Ultrastructural and Antigenic Features Simulating Dystrophic or Immunofluorescence-Negative Acquired Epidermolysis Bullosa

Anne Epstein, Sophia J. Hendrick, Ramon L. Sanchez, Alvin Jr Solomon, Jo David Fine

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We describe a child who began developing subepidermal blisters in the recipient sites of split-thickness skin grafts; this process has continued for almost a year and continues to spare nongrafted skin. Routine histologic and immunofluorescence mapping studies demonstrated this disorder to be a relatively noninflammatory one characterized by sub-lamina densa blister formation. Results of direct immunofluorescence were negative. By electron microscopy, anchoring fibrils were sparse in number and in some areas appeared malformed; otherwise, the basement membrane zone was morphologically unremarkable. Bullous pemphigoid antigen, laminin, type IV collagen, epidermolysis bullosa acquisita antigen, and LDA-1 were all normally expressed along the dermoepidermal junction. In contrast, KF-1 antigen was absent. These findings suggest a disease process confined to skin graft recipient sites with features identical to those previously described with recessive dystrophic or immunofluorescence-negative acquired epidermolysis bullosa.

Original languageEnglish (US)
Pages (from-to)244-249
Number of pages6
JournalArchives of Dermatology
Volume124
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Epidermolysis Bullosa Acquisita
Fluorescent Antibody Technique
Blister
Transplants
Skin
Antigens
Bullous Pemphigoid
Direct Fluorescent Antibody Technique
Collagen Type IV
Laminin
Basement Membrane
Electron Microscopy

ASJC Scopus subject areas

  • Dermatology

Cite this

Persistent Subepidermal Blistering in Split-Thickness Skin Graft Sites : Ultrastructural and Antigenic Features Simulating Dystrophic or Immunofluorescence-Negative Acquired Epidermolysis Bullosa. / Epstein, Anne; Hendrick, Sophia J.; Sanchez, Ramon L.; Solomon, Alvin Jr; Fine, Jo David.

In: Archives of Dermatology, Vol. 124, No. 2, 1988, p. 244-249.

Research output: Contribution to journalArticle

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