Persistent activation of min K channels by chemical cross-linking

Michael D. Varnum, James Maylie, Andreas Busch, John P. Adelman

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Expression of the structurally and functionally distinct min K channel in Xenopus oocytes results in voltage-dependent potassium currents that activate with a characteristic slow time course. Application of a membrane-impermeable chemical cross-linking agent to oocytes expressing min K decreased the time-dependent current, increased its rate of activation, and induced persistently activated inward and outward potassium currents. These effects required membrane depolarization, demonstrating use dependence. Persistently activated channels retained potassium selectivity and sensitivity to block by clofilium and barium. These results suggest that a major conformational change occurs during min K channel gating, which can be stabilized by chemical cross-linking, and are consistent with a model in which min K channels activate by voltage-dependent subunit aggregation.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalNeuron
Volume14
Issue number2
DOIs
StatePublished - Feb 1995

ASJC Scopus subject areas

  • Neuroscience(all)

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