@article{fad71326bf994415a1abb356463b1c36,
title = "Persistence of HIV-1 Env-Specific Plasmablast Lineages in Plasma Cells after Vaccination in Humans",
abstract = "In a phase I HIV vaccine trial, Basu et al. show the robust response of HIV Env gp120-specific peripheral blood plasmablasts immediately after vaccination, dominated by VH1 gene usage and V3 region-targeting Abs. They also define persistent linkage of these Env-reactive lineages to the bone marrow CD138+ LLPC compartment.",
keywords = "B cell, HIV-1, antibody, bone marrow, envelope, plasma cell, repertoire, vaccine",
author = "Madhubanti Basu and Piepenbrink, {Michael S.} and Czestochowa Francois and Fritzlaine Roche and Bo Zheng and Spencer, {David A.} and Hessell, {Ann J.} and Fucile, {Christopher F.} and Rosenberg, {Alexander F.} and Bunce, {Catherine A.} and Jane Liesveld and Keefer, {Michael C.} and Kobie, {James J.}",
note = "Funding Information: We are grateful for the assistance of the Rochester Victory Alliance and its staff. We are grateful for the technical assistance provided by Danielle DeLooze, Kevin Jin, Inho Cha, Claire Ruben, and Haien Son and for the assistance of the University of Rochester Flow Cytometry Core Facility and the University of Rochester Genomic Research Core. We are most grateful for the participation of the HVTN 105 study volunteers and thank the HVTN protocol team for their support of this project. We are very appreciative of the assistance provided by the HIV Vaccine Trials Network in enabling and coordinating this project, with particular acknowledgment of Cecilia Morgan. We are thankful to Dr. Kevin Saunders of Duke University for providing plasmid and technical advice and to Dr. Nancy Haigwood of Oregon Health & Science University-Oregon National Primate Research Center (OHSU-ONPRC) for helpful advice in preparing the manuscript. This research was partially funded by the National Institutes of Health (NIH) (5R21AI116285, 5R01AI117787, to J.J.K.; UM1AI069511, to M.C.K.; and UM1AI068614, to C.F. as part of the HVTN Research and Mentorship Program [RAMP]), and the University of Rochester Center for AIDS Research P30AI078498 (National Institute of Allergy and Infectious Diseases [NIAID]). M.B. C.A.B. A.F.R. A.J.H. J.L.L. M.C.K. and J.J.K. conceived and designed the study. M.B. M.S.P. C.F. F.R. B.Z. and D.S. performed the experiments. M.B. C.F.F. A.F.R. and J.J.K. performed the data curation and analysis. M.B. A.F.R. and J.J.K. drafted and edited the manuscript. The authors declare no competing interests. Funding Information: We are grateful for the assistance of the Rochester Victory Alliance and its staff. We are grateful for the technical assistance provided by Danielle DeLooze, Kevin Jin, Inho Cha, Claire Ruben, and Haien Son and for the assistance of the University of Rochester Flow Cytometry Core Facility and the University of Rochester Genomic Research Core. We are most grateful for the participation of the HVTN 105 study volunteers and thank the HVTN protocol team for their support of this project. We are very appreciative of the assistance provided by the HIV Vaccine Trials Network in enabling and coordinating this project, with particular acknowledgment of Cecilia Morgan. We are thankful to Dr. Kevin Saunders of Duke University for providing plasmid and technical advice and to Dr. Nancy Haigwood of Oregon Health & Science University-Oregon National Primate Research Center (OHSU-ONPRC) for helpful advice in preparing the manuscript. This research was partially funded by the National Institutes of Health (NIH) ( 5R21AI116285, 5R01AI117787 , to J.J.K.; UM1AI069511 , to M.C.K.; and UM1AI068614 , to C.F. as part of the HVTN Research and Mentorship Program [RAMP]), and the University of Rochester Center for AIDS Research P30AI078498 ( National Institute of Allergy and Infectious Diseases [NIAID]). Publisher Copyright: {\textcopyright} 2020 The Author(s) Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = may,
day = "19",
doi = "10.1016/j.xcrm.2020.100015",
language = "English (US)",
volume = "1",
journal = "Cell Reports Medicine",
issn = "2666-3791",
number = "2",
}