Persistence of chromatid aberrations in the cells of solid mouse tissues exposed to 137Cs gamma radiation

Mitchell Turker, Jeffrey L. Schwartz, Robert Jordan, Olga N. Ponomareva, Lanelle Connolly, Elizabeth Kasameyer, Michael Lasarev, Lisa Clepper

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Primary mouse ear and kidney cultures were established for determination of cytogenetic aberrations at short (3 days to 1 month) and long (12-23 months) times after exposure of their right sides to 7.5 Gy of 137Cs γ radiation. In every case, higher levels of aberrations were observed in primary cultures established from the irradiated tissues than in those established from the contralateral tissues. The most common aberrations in the contralateral tissues and those from nonirradiated mice were chromatid and isochromatid breaks and small chromatid fragments. Primary cells from irradiated tissues removed from animals within a month of exposure displayed a variety of unstable chromosome-type aberrations characteristic of recent exposure to ionizing radiation including rings, dicentrics, double minutes, and large acentric fragments. The percentages of cells exhibiting chromatid breaks and small chromatid fragments were also markedly elevated. Although the levels of chromosome-type aberrations found in primary cells from irradiated tissues dropped to near background levels a year or more after exposure, chromatid-type aberrations remained elevated. These results are consistent with long-term persistence of damage in the genomes of ionizing radiation-exposed cells in solid tissues and the induction of genomic instability in vivo.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalRadiation Research
Volume162
Issue number4
DOIs
StatePublished - Oct 2004

Fingerprint

Chromatids
chromatids
Gamma Rays
gamma radiation
mice
aberration
gamma rays
cells
Chromosome Aberrations
chromosomes
fragments
Ionizing Radiation
ionizing radiation
Radiation Dosage
chromosome aberrations
genome
Genomic Instability
kidneys
ear
Ear

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Turker, M., Schwartz, J. L., Jordan, R., Ponomareva, O. N., Connolly, L., Kasameyer, E., ... Clepper, L. (2004). Persistence of chromatid aberrations in the cells of solid mouse tissues exposed to 137Cs gamma radiation. Radiation Research, 162(4), 357-364. https://doi.org/10.1667/RR3240

Persistence of chromatid aberrations in the cells of solid mouse tissues exposed to 137Cs gamma radiation. / Turker, Mitchell; Schwartz, Jeffrey L.; Jordan, Robert; Ponomareva, Olga N.; Connolly, Lanelle; Kasameyer, Elizabeth; Lasarev, Michael; Clepper, Lisa.

In: Radiation Research, Vol. 162, No. 4, 10.2004, p. 357-364.

Research output: Contribution to journalArticle

Turker, M, Schwartz, JL, Jordan, R, Ponomareva, ON, Connolly, L, Kasameyer, E, Lasarev, M & Clepper, L 2004, 'Persistence of chromatid aberrations in the cells of solid mouse tissues exposed to 137Cs gamma radiation', Radiation Research, vol. 162, no. 4, pp. 357-364. https://doi.org/10.1667/RR3240
Turker, Mitchell ; Schwartz, Jeffrey L. ; Jordan, Robert ; Ponomareva, Olga N. ; Connolly, Lanelle ; Kasameyer, Elizabeth ; Lasarev, Michael ; Clepper, Lisa. / Persistence of chromatid aberrations in the cells of solid mouse tissues exposed to 137Cs gamma radiation. In: Radiation Research. 2004 ; Vol. 162, No. 4. pp. 357-364.
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