Peroxisome proliferator-activated receptor γcontrols ingestive behavior, agouti-related protein, and neuropeptide Y mRNA in the arcuate hypothalamus

Xjohn T. Garretson, Brett J W Teubner, Kevin Grove, Almira Vazdarjanova, Vitaly Ryu, Timothy J. Bartness

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Peroxisome proliferator-activated receptor γ (PPARγ) is clinically targeted for type II diabetes treatment; however, rosiglitazone (ROSI), a PPARγ agonist, increases food intake and body/fat mass as side-effects. Mechanisms for these effects and the role of PPARγ in feeding are not understood. Therefore, we tested this role in Siberian hamsters, a model of human energy balance, and C57BL/6 mice. We tested the following: (1) how ROSI and/or GW9662 (2-chloro-5-nitro-N-phenylbenzamide;PPARγ antagonist) injected intraperitoneally or into the third ventricle (3V) affected Siberian hamster feeding behaviors; (2) whether food deprivation (FD) co-increases agouti-related protein (AgRP) and PPARγ mRNAexpression in Siberian hamsters and mice; (3) whether intraperitoneally administered ROSI increases AgRP andNPYin ad libitum-fed animals; (4) whether intraperitoneally administeredPPARγ antagonism blocks FD-induced increases in AgRP and NPY; and finally, (5) whether intraperitoneally administered PPAR γ modulation affects plasma ghrelin. Third ventricular and intraperitoneally administered ROSI increased food hoarding and intake for 7 d, an effect attenuated by 3V GW9662, and also prevented (intraperitoneal) FD-induced feeding. FD hamsters and mice increased AgRP within the arcuate hypothalamic nucleus with concomitant increases in PPAR γ exclusively within AgRP/NPY neurons. ROSI increased AgRP and NPY similarly to FD, and GW9662 prevented FD-induced increases in AgRP and NPY in both species. Neither ROSI nor GW9662 affected plasma ghrelin. Thus, we demonstrated that PPAR γ activation is sufficient to trigger food hoarding/intake, increase AgRP/NPY, and possibly is necessary for FD-induced increases in feeding and AgRP/NPY. These findings provide initial evidence that FD-induced increases in AgRP/NPY may be a direct PPARγ-dependent process that controls ingestive behaviors.

    Original languageEnglish (US)
    Pages (from-to)4571-4581
    Number of pages11
    JournalJournal of Neuroscience
    Volume35
    Issue number11
    DOIs
    StatePublished - 2015

    Fingerprint

    Agouti-Related Protein
    rosiglitazone
    Peroxisome Proliferator-Activated Receptors
    Behavior Control
    Food Deprivation
    Neuropeptide Y
    Hypothalamus
    Messenger RNA
    Phodopus
    Ghrelin
    Eating
    Arcuate Nucleus of Hypothalamus
    Third Ventricle
    Feeding Behavior
    Inbred C57BL Mouse
    Cricetinae
    Type 2 Diabetes Mellitus
    Adipose Tissue

    Keywords

    • AgRP
    • Arcuate
    • Food hoarding
    • Ingestive behavior
    • NPY
    • PPAR gamma

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Peroxisome proliferator-activated receptor γcontrols ingestive behavior, agouti-related protein, and neuropeptide Y mRNA in the arcuate hypothalamus. / Garretson, Xjohn T.; Teubner, Brett J W; Grove, Kevin; Vazdarjanova, Almira; Ryu, Vitaly; Bartness, Timothy J.

    In: Journal of Neuroscience, Vol. 35, No. 11, 2015, p. 4571-4581.

    Research output: Contribution to journalArticle

    Garretson, Xjohn T. ; Teubner, Brett J W ; Grove, Kevin ; Vazdarjanova, Almira ; Ryu, Vitaly ; Bartness, Timothy J. / Peroxisome proliferator-activated receptor γcontrols ingestive behavior, agouti-related protein, and neuropeptide Y mRNA in the arcuate hypothalamus. In: Journal of Neuroscience. 2015 ; Vol. 35, No. 11. pp. 4571-4581.
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    abstract = "Peroxisome proliferator-activated receptor γ (PPARγ) is clinically targeted for type II diabetes treatment; however, rosiglitazone (ROSI), a PPARγ agonist, increases food intake and body/fat mass as side-effects. Mechanisms for these effects and the role of PPARγ in feeding are not understood. Therefore, we tested this role in Siberian hamsters, a model of human energy balance, and C57BL/6 mice. We tested the following: (1) how ROSI and/or GW9662 (2-chloro-5-nitro-N-phenylbenzamide;PPARγ antagonist) injected intraperitoneally or into the third ventricle (3V) affected Siberian hamster feeding behaviors; (2) whether food deprivation (FD) co-increases agouti-related protein (AgRP) and PPARγ mRNAexpression in Siberian hamsters and mice; (3) whether intraperitoneally administered ROSI increases AgRP andNPYin ad libitum-fed animals; (4) whether intraperitoneally administeredPPARγ antagonism blocks FD-induced increases in AgRP and NPY; and finally, (5) whether intraperitoneally administered PPAR γ modulation affects plasma ghrelin. Third ventricular and intraperitoneally administered ROSI increased food hoarding and intake for 7 d, an effect attenuated by 3V GW9662, and also prevented (intraperitoneal) FD-induced feeding. FD hamsters and mice increased AgRP within the arcuate hypothalamic nucleus with concomitant increases in PPAR γ exclusively within AgRP/NPY neurons. ROSI increased AgRP and NPY similarly to FD, and GW9662 prevented FD-induced increases in AgRP and NPY in both species. Neither ROSI nor GW9662 affected plasma ghrelin. Thus, we demonstrated that PPAR γ activation is sufficient to trigger food hoarding/intake, increase AgRP/NPY, and possibly is necessary for FD-induced increases in feeding and AgRP/NPY. These findings provide initial evidence that FD-induced increases in AgRP/NPY may be a direct PPARγ-dependent process that controls ingestive behaviors.",
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    AU - Grove, Kevin

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    AU - Ryu, Vitaly

    AU - Bartness, Timothy J.

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