Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor: A potential role for T cells in angiogenesis

M. R. Freeman, F. X. Schneck, M. L. Gagnon, C. Corless, S. Soker, K. Niknejad, G. E. Peoples, Christopher Corless

Research output: Contribution to journalArticle

366 Citations (Scopus)

Abstract

CD3+ peripheral blood T lymphocytes were evaluated for expression of vascular endothelial growth factor (VEGF), an endothelial cell mitogen and potent angiogenic factor. VEGF mRNA expression was confirmed in CD3+ cells and Jurkat cells, a human T-cell line, by reverse transcription-PCR and in CD4+ and CD8+ T cell subtypes by Northern blot hybridization. Steady-state levels of VEGF mRNA were inducible in CD3+ T cells by hypoxia, a known inducer of VEGF mRNA accumulation. Secreted VEGF was detected in CD4+ and CD8+ T cell- and Jurkat cell-conditioned medium, indicating that T lymphocytes are capable of exporting bioactive concentrations of VEGF into the extracellular space. Human prostate and bladder cancers (prostatic adenocarcinoma and transitional cell carcinomas) were evaluated for VEGF mRNA expression by in situ hybridization. Tumor-infiltrating lymphocytes (TIL), identifiable immunocytochemically as T cells, along with tumor cells in these cancers, expressed VEGF mRNA. TIL in bladder cancers could be labeled with a specific anti-VEGF mAb, indicating that TIL are likely to be able to secrete VEGF protein in situ at bioactive concentrations. The finding that peripheral T cells and TIL in human tumors synthesize a factor known to be a specific mediator of neovascularization suggests a role for T lymphocytes as cellular effectors of angiogenesis.

Original languageEnglish (US)
Pages (from-to)4140-4145
Number of pages6
JournalCancer Research
Volume55
Issue number18
StatePublished - 1995
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor A
Lymphocytes
T-Lymphocytes
Tumor-Infiltrating Lymphocytes
Neoplasms
Messenger RNA
Jurkat Cells
Urinary Bladder Neoplasms
Cell Hypoxia
Transitional Cell Carcinoma
Angiogenesis Inducing Agents
Extracellular Space
Conditioned Culture Medium
Mitogens
Northern Blotting
Reverse Transcription
In Situ Hybridization
Prostatic Neoplasms
Adenocarcinoma
Endothelial Cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor : A potential role for T cells in angiogenesis. / Freeman, M. R.; Schneck, F. X.; Gagnon, M. L.; Corless, C.; Soker, S.; Niknejad, K.; Peoples, G. E.; Corless, Christopher.

In: Cancer Research, Vol. 55, No. 18, 1995, p. 4140-4145.

Research output: Contribution to journalArticle

Freeman, MR, Schneck, FX, Gagnon, ML, Corless, C, Soker, S, Niknejad, K, Peoples, GE & Corless, C 1995, 'Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor: A potential role for T cells in angiogenesis', Cancer Research, vol. 55, no. 18, pp. 4140-4145.
Freeman, M. R. ; Schneck, F. X. ; Gagnon, M. L. ; Corless, C. ; Soker, S. ; Niknejad, K. ; Peoples, G. E. ; Corless, Christopher. / Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor : A potential role for T cells in angiogenesis. In: Cancer Research. 1995 ; Vol. 55, No. 18. pp. 4140-4145.
@article{80e6afcb10f74803acfb6ea60c1badbb,
title = "Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor: A potential role for T cells in angiogenesis",
abstract = "CD3+ peripheral blood T lymphocytes were evaluated for expression of vascular endothelial growth factor (VEGF), an endothelial cell mitogen and potent angiogenic factor. VEGF mRNA expression was confirmed in CD3+ cells and Jurkat cells, a human T-cell line, by reverse transcription-PCR and in CD4+ and CD8+ T cell subtypes by Northern blot hybridization. Steady-state levels of VEGF mRNA were inducible in CD3+ T cells by hypoxia, a known inducer of VEGF mRNA accumulation. Secreted VEGF was detected in CD4+ and CD8+ T cell- and Jurkat cell-conditioned medium, indicating that T lymphocytes are capable of exporting bioactive concentrations of VEGF into the extracellular space. Human prostate and bladder cancers (prostatic adenocarcinoma and transitional cell carcinomas) were evaluated for VEGF mRNA expression by in situ hybridization. Tumor-infiltrating lymphocytes (TIL), identifiable immunocytochemically as T cells, along with tumor cells in these cancers, expressed VEGF mRNA. TIL in bladder cancers could be labeled with a specific anti-VEGF mAb, indicating that TIL are likely to be able to secrete VEGF protein in situ at bioactive concentrations. The finding that peripheral T cells and TIL in human tumors synthesize a factor known to be a specific mediator of neovascularization suggests a role for T lymphocytes as cellular effectors of angiogenesis.",
author = "Freeman, {M. R.} and Schneck, {F. X.} and Gagnon, {M. L.} and C. Corless and S. Soker and K. Niknejad and Peoples, {G. E.} and Christopher Corless",
year = "1995",
language = "English (US)",
volume = "55",
pages = "4140--4145",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "18",

}

TY - JOUR

T1 - Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor

T2 - A potential role for T cells in angiogenesis

AU - Freeman, M. R.

AU - Schneck, F. X.

AU - Gagnon, M. L.

AU - Corless, C.

AU - Soker, S.

AU - Niknejad, K.

AU - Peoples, G. E.

AU - Corless, Christopher

PY - 1995

Y1 - 1995

N2 - CD3+ peripheral blood T lymphocytes were evaluated for expression of vascular endothelial growth factor (VEGF), an endothelial cell mitogen and potent angiogenic factor. VEGF mRNA expression was confirmed in CD3+ cells and Jurkat cells, a human T-cell line, by reverse transcription-PCR and in CD4+ and CD8+ T cell subtypes by Northern blot hybridization. Steady-state levels of VEGF mRNA were inducible in CD3+ T cells by hypoxia, a known inducer of VEGF mRNA accumulation. Secreted VEGF was detected in CD4+ and CD8+ T cell- and Jurkat cell-conditioned medium, indicating that T lymphocytes are capable of exporting bioactive concentrations of VEGF into the extracellular space. Human prostate and bladder cancers (prostatic adenocarcinoma and transitional cell carcinomas) were evaluated for VEGF mRNA expression by in situ hybridization. Tumor-infiltrating lymphocytes (TIL), identifiable immunocytochemically as T cells, along with tumor cells in these cancers, expressed VEGF mRNA. TIL in bladder cancers could be labeled with a specific anti-VEGF mAb, indicating that TIL are likely to be able to secrete VEGF protein in situ at bioactive concentrations. The finding that peripheral T cells and TIL in human tumors synthesize a factor known to be a specific mediator of neovascularization suggests a role for T lymphocytes as cellular effectors of angiogenesis.

AB - CD3+ peripheral blood T lymphocytes were evaluated for expression of vascular endothelial growth factor (VEGF), an endothelial cell mitogen and potent angiogenic factor. VEGF mRNA expression was confirmed in CD3+ cells and Jurkat cells, a human T-cell line, by reverse transcription-PCR and in CD4+ and CD8+ T cell subtypes by Northern blot hybridization. Steady-state levels of VEGF mRNA were inducible in CD3+ T cells by hypoxia, a known inducer of VEGF mRNA accumulation. Secreted VEGF was detected in CD4+ and CD8+ T cell- and Jurkat cell-conditioned medium, indicating that T lymphocytes are capable of exporting bioactive concentrations of VEGF into the extracellular space. Human prostate and bladder cancers (prostatic adenocarcinoma and transitional cell carcinomas) were evaluated for VEGF mRNA expression by in situ hybridization. Tumor-infiltrating lymphocytes (TIL), identifiable immunocytochemically as T cells, along with tumor cells in these cancers, expressed VEGF mRNA. TIL in bladder cancers could be labeled with a specific anti-VEGF mAb, indicating that TIL are likely to be able to secrete VEGF protein in situ at bioactive concentrations. The finding that peripheral T cells and TIL in human tumors synthesize a factor known to be a specific mediator of neovascularization suggests a role for T lymphocytes as cellular effectors of angiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0029085337&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029085337&partnerID=8YFLogxK

M3 - Article

C2 - 7545086

AN - SCOPUS:0029085337

VL - 55

SP - 4140

EP - 4145

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 18

ER -