Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids

Joel L. Ramirez, Greg J. Zahner, Kimberly A. Spaulding, Sukaynah A. Khetani, Nancy K. Hills, Warren J. Gasper, William Harris, Beth E. Cohen, S. Marlene Grenon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Population-based data suggest that individuals who consume large dietary amounts of n-3 polyunsaturated fatty acids (PUFA) have lower odds of peripheral artery disease (PAD); however, clinical studies examining n-3 PUFA levels in patients with PAD are sparse. The objective of this study is to compare erythrocyte membrane fatty acid (FA) content between patients with PAD and controls. We conducted a cross-sectional study of 179 vascular surgery outpatients (controls, 34; PAD, 145). A blood sample was drawn and the erythrocyte FA content was assayed using capillary gas chromatography. We calculated the ratio of the n-3 PUFA eicosapentaenoic acid (EPA) to the n-6 PUFA arachidonic acid (ARA) as well as the omega-3 index (O3I), a measure of erythrocyte content of the n-3 PUFA, EPA, and docosahexaenoic acid (DHA), expressed as a percentage of total erythrocyte FA. Compared with controls, patients with PAD smoked more and were more likely to have hypertension and hyperlipidemia (p < 0.05). Patients with PAD had a lower mean O3I (5.0 ± 1.7% vs 6.0 ± 1.6%, p < 0.001) and EPA:ARA ratio (0.04 ± 0.02 vs 0.05 ± 0.05, p < 0.001), but greater mean total saturated fats (39.5 ± 2.5% vs 38.5 ± 2.6%, p = 0.01). After adjusting for several patient characteristics, comorbidities, and medications, an absolute decrease of 1% in the O3I was associated with 39% greater odds of PAD (odds ratio [OR] 1.39, 95% confidence interval [CI] 1.03–1.86, and p = 0.03). PAD was associated with a deficiency of erythrocyte n-3 PUFA, a lower EPA:ARA ratio, and greater mean total saturated fats. These alterations in FA content may be involved in the pathogenesis or development of poor outcomes in PAD.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalLipids
Volume54
Issue number4
DOIs
StatePublished - Apr 1 2019
Externally publishedYes

Fingerprint

Peripheral Arterial Disease
Omega-3 Fatty Acids
Erythrocyte Membrane
Membranes
Eicosapentaenoic Acid
Fatty Acids
Erythrocytes
Arachidonic Acid
Fats
Docosahexaenoic Acids
Hyperlipidemias
Ambulatory Surgical Procedures
Unsaturated Fatty Acids
Gas chromatography
Gas Chromatography
Surgery
Blood Vessels
Comorbidity
Blood
Cross-Sectional Studies

Keywords

  • Atherosclerosis
  • Lipid analysis
  • n-3 fatty acids

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Ramirez, J. L., Zahner, G. J., Spaulding, K. A., Khetani, S. A., Hills, N. K., Gasper, W. J., ... Grenon, S. M. (2019). Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids. Lipids, 54(4), 211-219. https://doi.org/10.1002/lipd.12140

Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids. / Ramirez, Joel L.; Zahner, Greg J.; Spaulding, Kimberly A.; Khetani, Sukaynah A.; Hills, Nancy K.; Gasper, Warren J.; Harris, William; Cohen, Beth E.; Grenon, S. Marlene.

In: Lipids, Vol. 54, No. 4, 01.04.2019, p. 211-219.

Research output: Contribution to journalArticle

Ramirez, JL, Zahner, GJ, Spaulding, KA, Khetani, SA, Hills, NK, Gasper, WJ, Harris, W, Cohen, BE & Grenon, SM 2019, 'Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids', Lipids, vol. 54, no. 4, pp. 211-219. https://doi.org/10.1002/lipd.12140
Ramirez JL, Zahner GJ, Spaulding KA, Khetani SA, Hills NK, Gasper WJ et al. Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids. Lipids. 2019 Apr 1;54(4):211-219. https://doi.org/10.1002/lipd.12140
Ramirez, Joel L. ; Zahner, Greg J. ; Spaulding, Kimberly A. ; Khetani, Sukaynah A. ; Hills, Nancy K. ; Gasper, Warren J. ; Harris, William ; Cohen, Beth E. ; Grenon, S. Marlene. / Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids. In: Lipids. 2019 ; Vol. 54, No. 4. pp. 211-219.
@article{b563c240325e4c068c5c1125409018e6,
title = "Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids",
abstract = "Population-based data suggest that individuals who consume large dietary amounts of n-3 polyunsaturated fatty acids (PUFA) have lower odds of peripheral artery disease (PAD); however, clinical studies examining n-3 PUFA levels in patients with PAD are sparse. The objective of this study is to compare erythrocyte membrane fatty acid (FA) content between patients with PAD and controls. We conducted a cross-sectional study of 179 vascular surgery outpatients (controls, 34; PAD, 145). A blood sample was drawn and the erythrocyte FA content was assayed using capillary gas chromatography. We calculated the ratio of the n-3 PUFA eicosapentaenoic acid (EPA) to the n-6 PUFA arachidonic acid (ARA) as well as the omega-3 index (O3I), a measure of erythrocyte content of the n-3 PUFA, EPA, and docosahexaenoic acid (DHA), expressed as a percentage of total erythrocyte FA. Compared with controls, patients with PAD smoked more and were more likely to have hypertension and hyperlipidemia (p < 0.05). Patients with PAD had a lower mean O3I (5.0 ± 1.7{\%} vs 6.0 ± 1.6{\%}, p < 0.001) and EPA:ARA ratio (0.04 ± 0.02 vs 0.05 ± 0.05, p < 0.001), but greater mean total saturated fats (39.5 ± 2.5{\%} vs 38.5 ± 2.6{\%}, p = 0.01). After adjusting for several patient characteristics, comorbidities, and medications, an absolute decrease of 1{\%} in the O3I was associated with 39{\%} greater odds of PAD (odds ratio [OR] 1.39, 95{\%} confidence interval [CI] 1.03–1.86, and p = 0.03). PAD was associated with a deficiency of erythrocyte n-3 PUFA, a lower EPA:ARA ratio, and greater mean total saturated fats. These alterations in FA content may be involved in the pathogenesis or development of poor outcomes in PAD.",
keywords = "Atherosclerosis, Lipid analysis, n-3 fatty acids",
author = "Ramirez, {Joel L.} and Zahner, {Greg J.} and Spaulding, {Kimberly A.} and Khetani, {Sukaynah A.} and Hills, {Nancy K.} and Gasper, {Warren J.} and William Harris and Cohen, {Beth E.} and Grenon, {S. Marlene}",
year = "2019",
month = "4",
day = "1",
doi = "10.1002/lipd.12140",
language = "English (US)",
volume = "54",
pages = "211--219",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Peripheral Artery Disease Is Associated with a Deficiency of Erythrocyte Membrane n-3 Polyunsaturated Fatty Acids

AU - Ramirez, Joel L.

AU - Zahner, Greg J.

AU - Spaulding, Kimberly A.

AU - Khetani, Sukaynah A.

AU - Hills, Nancy K.

AU - Gasper, Warren J.

AU - Harris, William

AU - Cohen, Beth E.

AU - Grenon, S. Marlene

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Population-based data suggest that individuals who consume large dietary amounts of n-3 polyunsaturated fatty acids (PUFA) have lower odds of peripheral artery disease (PAD); however, clinical studies examining n-3 PUFA levels in patients with PAD are sparse. The objective of this study is to compare erythrocyte membrane fatty acid (FA) content between patients with PAD and controls. We conducted a cross-sectional study of 179 vascular surgery outpatients (controls, 34; PAD, 145). A blood sample was drawn and the erythrocyte FA content was assayed using capillary gas chromatography. We calculated the ratio of the n-3 PUFA eicosapentaenoic acid (EPA) to the n-6 PUFA arachidonic acid (ARA) as well as the omega-3 index (O3I), a measure of erythrocyte content of the n-3 PUFA, EPA, and docosahexaenoic acid (DHA), expressed as a percentage of total erythrocyte FA. Compared with controls, patients with PAD smoked more and were more likely to have hypertension and hyperlipidemia (p < 0.05). Patients with PAD had a lower mean O3I (5.0 ± 1.7% vs 6.0 ± 1.6%, p < 0.001) and EPA:ARA ratio (0.04 ± 0.02 vs 0.05 ± 0.05, p < 0.001), but greater mean total saturated fats (39.5 ± 2.5% vs 38.5 ± 2.6%, p = 0.01). After adjusting for several patient characteristics, comorbidities, and medications, an absolute decrease of 1% in the O3I was associated with 39% greater odds of PAD (odds ratio [OR] 1.39, 95% confidence interval [CI] 1.03–1.86, and p = 0.03). PAD was associated with a deficiency of erythrocyte n-3 PUFA, a lower EPA:ARA ratio, and greater mean total saturated fats. These alterations in FA content may be involved in the pathogenesis or development of poor outcomes in PAD.

AB - Population-based data suggest that individuals who consume large dietary amounts of n-3 polyunsaturated fatty acids (PUFA) have lower odds of peripheral artery disease (PAD); however, clinical studies examining n-3 PUFA levels in patients with PAD are sparse. The objective of this study is to compare erythrocyte membrane fatty acid (FA) content between patients with PAD and controls. We conducted a cross-sectional study of 179 vascular surgery outpatients (controls, 34; PAD, 145). A blood sample was drawn and the erythrocyte FA content was assayed using capillary gas chromatography. We calculated the ratio of the n-3 PUFA eicosapentaenoic acid (EPA) to the n-6 PUFA arachidonic acid (ARA) as well as the omega-3 index (O3I), a measure of erythrocyte content of the n-3 PUFA, EPA, and docosahexaenoic acid (DHA), expressed as a percentage of total erythrocyte FA. Compared with controls, patients with PAD smoked more and were more likely to have hypertension and hyperlipidemia (p < 0.05). Patients with PAD had a lower mean O3I (5.0 ± 1.7% vs 6.0 ± 1.6%, p < 0.001) and EPA:ARA ratio (0.04 ± 0.02 vs 0.05 ± 0.05, p < 0.001), but greater mean total saturated fats (39.5 ± 2.5% vs 38.5 ± 2.6%, p = 0.01). After adjusting for several patient characteristics, comorbidities, and medications, an absolute decrease of 1% in the O3I was associated with 39% greater odds of PAD (odds ratio [OR] 1.39, 95% confidence interval [CI] 1.03–1.86, and p = 0.03). PAD was associated with a deficiency of erythrocyte n-3 PUFA, a lower EPA:ARA ratio, and greater mean total saturated fats. These alterations in FA content may be involved in the pathogenesis or development of poor outcomes in PAD.

KW - Atherosclerosis

KW - Lipid analysis

KW - n-3 fatty acids

UR - http://www.scopus.com/inward/record.url?scp=85064827641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064827641&partnerID=8YFLogxK

U2 - 10.1002/lipd.12140

DO - 10.1002/lipd.12140

M3 - Article

VL - 54

SP - 211

EP - 219

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 4

ER -