Percutaneous absorption and model membrane variations of melatonin in aqueous-based propylene glycol and 2-hydroxypropyl-β-cyclodextrin vehicles

Beom Jin Lee, Jing Hao Cui, Keith A. Parrott, James W. Ayres, Robert Sack

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Percutaneous absorption and model membrane variations of melatonin (MT) in aqueous-based propylene glycol and 2-hydroxypropyl-β-cyclodextrin vehicles were investigated. The excised hairless mouse skin (HMS) and two synthetic ethylene vinyl acetate (EVA) and microporous polyethylene (MPE) were selected as a model membrane. The solubility of MT was determined by phase equilibrium study. The vertical Franz® type cell was used for diffusion study. The concentration of MT was determined using reverse phase HPLC system. The MT solubility was the highest in a mixture of PG and 2-HPβCD. The percutaneous absorption of MT through excised HMS increased as the solubility increased. However, the permeability coefficient decreased and then slightly increased in a mixture of PG and 2-HPβCD. On the other hand, both flux and permeability coefficient through EVA membrane decreased as the solubility increased. No MT was detected over 12 h after starting diffusion through MPE membrane. The flux of MT was dependent on the type of membrane selected. Flux of MT was greatest in excised HMS followed by EVA and MPE membrane. Flux of MT through EVA membrane was 5-20 times lower when compared to excised HMS. Interestingly, volumes of donor phase when MPE membrane was used, significantly increased during the study period. The HMS might be applicable to expect plasma concentration of MT in human subjects based on flux and pharmacokinetic parameters as studied previously. The current studies may be applied to deliver MT transdermally using aqueous-based vehicles and to fabricate MT dosage forms.

Original languageEnglish (US)
Pages (from-to)503-507
Number of pages5
JournalArchives of Pharmacal Research
Volume21
Issue number5
StatePublished - Oct 1998

Fingerprint

Skin Absorption
Propylene Glycol
Cyclodextrins
Melatonin
Membranes
Hairless Mouse
Skin
Solubility
Fluxes
Hydraulic conductivity
Polyethylene
Permeability
Pharmacokinetics
Dosage Forms
Phase equilibria

Keywords

  • 2-Hydroxypropyl-β-cyclodextrin
  • Aqueous-based vehicles
  • Melatonin
  • Model membrane variations
  • Percutaneous absorption
  • Propylene glycol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Percutaneous absorption and model membrane variations of melatonin in aqueous-based propylene glycol and 2-hydroxypropyl-β-cyclodextrin vehicles. / Lee, Beom Jin; Cui, Jing Hao; Parrott, Keith A.; Ayres, James W.; Sack, Robert.

In: Archives of Pharmacal Research, Vol. 21, No. 5, 10.1998, p. 503-507.

Research output: Contribution to journalArticle

Lee, Beom Jin ; Cui, Jing Hao ; Parrott, Keith A. ; Ayres, James W. ; Sack, Robert. / Percutaneous absorption and model membrane variations of melatonin in aqueous-based propylene glycol and 2-hydroxypropyl-β-cyclodextrin vehicles. In: Archives of Pharmacal Research. 1998 ; Vol. 21, No. 5. pp. 503-507.
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