Peptides of a Novel Mycobacterium tuberculosis-Specific cell wall protein for immunodiagnosis of tuberculosis

Krishna K. Singh, Naresh Sharma, Diana Vargas, Zhentong Liu, John T. Belisle, Visalakshi Potharaju, Ajay Wanchu, Digambar Behera, Suman Laal

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The sequencing of the Mycobacterium tuberculosis genome revealed the existence of several genes encoding novel proteins with unknown functions, one of which is the proline-threonine repetitive protein (PTRP; Rv0538). Genomic studies of various mycobacterial species and M. tuberculosis clinical isolates demonstrate that ptrp is specific to the M. tuberculosis complex and ubiquitous in clinical isolates. Enzyme-linked immunosorbent assay, Western blot analysis, and electron microscopic evaluation of M. tuberculosis subcellular fractions and intact bacteria confirm that PTRP is a cell wall protein. Antibodies to PTRP are present in serum specimens from human immunodeficiency virus (HlV)-negative, tuberculosis (TB)-positive and HIVpositive, TB-positive patients but not purified protein derivative (PPD)-negative or PPD-positive healthy control subjects, demonstrating its diagnostic potential. Epitope mapping of PTRP delineated 4 peptides that can identify >80% of sputum smear-positive and >50% of smear-negative, HIV-negative, TB-positive patients and >80% of HIV-positive, TB-positive patients. These results demonstrate that immunodominant epitopes of carefully selected M. tuberculosis - specific proteins can be used to devise a simple peptide-based serodiagnostic test for TB.

Original languageEnglish (US)
Pages (from-to)571-581
Number of pages11
JournalJournal of Infectious Diseases
Volume200
Issue number4
DOIs
StatePublished - Aug 15 2009

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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