Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells

Frances M. Saccoccio, Anne L. Sauer, Xiaohong Cui, Amy E. Armstrong, EL Sayed E Habib, David Johnson, Brent J. Ryckman, Aloysius J. Klingelhutz, Stuart P. Adler, Michael A. McVoy

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.

Original languageEnglish (US)
Pages (from-to)2705-2711
Number of pages7
JournalVaccine
Volume29
Issue number15
DOIs
StatePublished - Mar 24 2011

Fingerprint

Viral Antibodies
Cytomegalovirus
epithelial cells
Epithelial Cells
peptides
Peptides
neutralization
genitalia
antibodies
mucosa
mouth
Proteins
Vaccines
proteins
vaccines
Retinal Pigment Epithelium
Antibodies
Cytomegalovirus Infections
Mouth Mucosa
Virus Diseases

Keywords

  • Cytomegalovirus
  • Mucosal infection
  • Neutralizing antibody
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells. / Saccoccio, Frances M.; Sauer, Anne L.; Cui, Xiaohong; Armstrong, Amy E.; Habib, EL Sayed E; Johnson, David; Ryckman, Brent J.; Klingelhutz, Aloysius J.; Adler, Stuart P.; McVoy, Michael A.

In: Vaccine, Vol. 29, No. 15, 24.03.2011, p. 2705-2711.

Research output: Contribution to journalArticle

Saccoccio, FM, Sauer, AL, Cui, X, Armstrong, AE, Habib, ELSE, Johnson, D, Ryckman, BJ, Klingelhutz, AJ, Adler, SP & McVoy, MA 2011, 'Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells', Vaccine, vol. 29, no. 15, pp. 2705-2711. https://doi.org/10.1016/j.vaccine.2011.01.079
Saccoccio, Frances M. ; Sauer, Anne L. ; Cui, Xiaohong ; Armstrong, Amy E. ; Habib, EL Sayed E ; Johnson, David ; Ryckman, Brent J. ; Klingelhutz, Aloysius J. ; Adler, Stuart P. ; McVoy, Michael A. / Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells. In: Vaccine. 2011 ; Vol. 29, No. 15. pp. 2705-2711.
@article{23d318dbc234424598cff21e02ad88df,
title = "Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells",
abstract = "Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.",
keywords = "Cytomegalovirus, Mucosal infection, Neutralizing antibody, Vaccine",
author = "Saccoccio, {Frances M.} and Sauer, {Anne L.} and Xiaohong Cui and Armstrong, {Amy E.} and Habib, {EL Sayed E} and David Johnson and Ryckman, {Brent J.} and Klingelhutz, {Aloysius J.} and Adler, {Stuart P.} and McVoy, {Michael A.}",
year = "2011",
month = "3",
day = "24",
doi = "10.1016/j.vaccine.2011.01.079",
language = "English (US)",
volume = "29",
pages = "2705--2711",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "15",

}

TY - JOUR

T1 - Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells

AU - Saccoccio, Frances M.

AU - Sauer, Anne L.

AU - Cui, Xiaohong

AU - Armstrong, Amy E.

AU - Habib, EL Sayed E

AU - Johnson, David

AU - Ryckman, Brent J.

AU - Klingelhutz, Aloysius J.

AU - Adler, Stuart P.

AU - McVoy, Michael A.

PY - 2011/3/24

Y1 - 2011/3/24

N2 - Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.

AB - Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.

KW - Cytomegalovirus

KW - Mucosal infection

KW - Neutralizing antibody

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=79952244625&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952244625&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2011.01.079

DO - 10.1016/j.vaccine.2011.01.079

M3 - Article

C2 - 21310190

AN - SCOPUS:79952244625

VL - 29

SP - 2705

EP - 2711

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 15

ER -