Peptide YY. Structure of the precursor and expression in exocrine pancreas.

A. B. Leiter, A. Toder, H. J. Wolfe, I. L. Taylor, S. Cooperman, Gail Mandel, Richard Goodman

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Peptide YY is a 36-residue gastrointestinal hormone which inhibits both pancreatic and gastric secretion. We have isolated a cDNA encoding the peptide YY precursor by screening a rat intestinal lambda gt11 cDNA library with an antiserum directed against the porcine hormone. The nucleotide sequence of the cDNA encodes a 98-residue protein (molecular weight, 11, 121) which has an amino acid sequence identical to that of porcine peptide YY. Rat peptide YY is preceded immediately by a signal sequence and followed by a cleavage-amidation sequence Gly-Lys-Arg plus 31 additional amino acids. Thus the peptide YY precursor is similar in structure to that of two related peptides, pancreatic polypeptide and neuropeptide Y. RNA blot hybridizations reveal that the peptide YY gene is much more actively expressed in pancreas than previously realized. In situ hybridizations localized peptide YY cells exclusively to the exocrine pancreas. The abundance of peptide YY in one of its target organs, the pancreas, suggests a paracrine mechanism for peptide YY in regulating pancreatic enzyme secretion.

Original languageEnglish (US)
Pages (from-to)12984-12988
Number of pages5
JournalJournal of Biological Chemistry
Volume262
Issue number27
StatePublished - Sep 25 1987
Externally publishedYes

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Peptide YY
Exocrine Pancreas
Rats
Pancreas
Swine
Complementary DNA
Gastrointestinal Hormones
Pancreatic Polypeptide
Amino Acids
Neuropeptide Y
Protein Sorting Signals
Gene Library
In Situ Hybridization
Immune Sera
Amino Acid Sequence
Stomach
Screening
Nucleotides
Genes
Molecular Weight

ASJC Scopus subject areas

  • Biochemistry

Cite this

Leiter, A. B., Toder, A., Wolfe, H. J., Taylor, I. L., Cooperman, S., Mandel, G., & Goodman, R. (1987). Peptide YY. Structure of the precursor and expression in exocrine pancreas. Journal of Biological Chemistry, 262(27), 12984-12988.

Peptide YY. Structure of the precursor and expression in exocrine pancreas. / Leiter, A. B.; Toder, A.; Wolfe, H. J.; Taylor, I. L.; Cooperman, S.; Mandel, Gail; Goodman, Richard.

In: Journal of Biological Chemistry, Vol. 262, No. 27, 25.09.1987, p. 12984-12988.

Research output: Contribution to journalArticle

Leiter, AB, Toder, A, Wolfe, HJ, Taylor, IL, Cooperman, S, Mandel, G & Goodman, R 1987, 'Peptide YY. Structure of the precursor and expression in exocrine pancreas.', Journal of Biological Chemistry, vol. 262, no. 27, pp. 12984-12988.
Leiter AB, Toder A, Wolfe HJ, Taylor IL, Cooperman S, Mandel G et al. Peptide YY. Structure of the precursor and expression in exocrine pancreas. Journal of Biological Chemistry. 1987 Sep 25;262(27):12984-12988.
Leiter, A. B. ; Toder, A. ; Wolfe, H. J. ; Taylor, I. L. ; Cooperman, S. ; Mandel, Gail ; Goodman, Richard. / Peptide YY. Structure of the precursor and expression in exocrine pancreas. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 27. pp. 12984-12988.
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