Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth

Tothu (Tania) Vu, Ravikanth Maddipati, Todd A. Blute, Barrett J. Nehilla, Leora Nusblat, Tejal A. Desai

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.

Original languageEnglish (US)
Pages (from-to)603-607
Number of pages5
JournalNano Letters
Volume5
Issue number4
DOIs
StatePublished - Apr 2005
Externally publishedYes

Fingerprint

Peptides
Semiconductor quantum dots
peptides
quantum dots
Nerve Growth Factor
trkA Receptor
cells
Ligands
physiological responses
ligands
Bioactivity
Scaffolds
Surface treatment
Nanostructures
atomic force microscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

Cite this

Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth. / Vu, Tothu (Tania); Maddipati, Ravikanth; Blute, Todd A.; Nehilla, Barrett J.; Nusblat, Leora; Desai, Tejal A.

In: Nano Letters, Vol. 5, No. 4, 04.2005, p. 603-607.

Research output: Contribution to journalArticle

Vu, Tothu (Tania) ; Maddipati, Ravikanth ; Blute, Todd A. ; Nehilla, Barrett J. ; Nusblat, Leora ; Desai, Tejal A. / Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth. In: Nano Letters. 2005 ; Vol. 5, No. 4. pp. 603-607.
@article{9c9aca60a774461da86490934ec2f120,
title = "Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth",
abstract = "Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.",
author = "Vu, {Tothu (Tania)} and Ravikanth Maddipati and Blute, {Todd A.} and Nehilla, {Barrett J.} and Leora Nusblat and Desai, {Tejal A.}",
year = "2005",
month = "4",
doi = "10.1021/nl047977c",
language = "English (US)",
volume = "5",
pages = "603--607",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "4",

}

TY - JOUR

T1 - Peptide-conjugated quantum dots activate neuronal receptors and initiate downstream signaling of neurite growth

AU - Vu, Tothu (Tania)

AU - Maddipati, Ravikanth

AU - Blute, Todd A.

AU - Nehilla, Barrett J.

AU - Nusblat, Leora

AU - Desai, Tejal A.

PY - 2005/4

Y1 - 2005/4

N2 - Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.

AB - Quantum dots (QDs) could serve as fluorescent scaffolds for effecting specific physiological and pharmacological responses in cells. Here, we conjugate the peptide ligand βNGF to QD surfaces, and confirm surface modification and single QD nanostructure using AFM. We show that βNGF-QDs retain bioactivity, activate TrkA receptors, and initiate neuronal differentiation in PC12 cells. Receptor-evoked activity of QD-immobilized ligands has wide-ranging implications for the development of molecular tools and therapeutics targeted at understanding and regulating cell function.

UR - http://www.scopus.com/inward/record.url?scp=18144391953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18144391953&partnerID=8YFLogxK

U2 - 10.1021/nl047977c

DO - 10.1021/nl047977c

M3 - Article

VL - 5

SP - 603

EP - 607

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 4

ER -