Peptide and lipid growth factors decrease cis-diamminedichloroplatinum- induced cell death in human ovarian cancer cells

Andrea Frankel, Gordon Mills

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Growth factors have been demonstrated to regulate the proliferation and viability of a number of cell lineages. Because most drugs used in chemotherapy kill cells through programmed cell death, by the process of apoptosis, we determined whether growth factors, specifically epidermal growth factor (EGF) and lysophosphatidic acid (LPA), which we have demonstrated recently to be a potent growth factor for ovarian cancer cells, would alter the ability of cis-diamminedichloroplatinum (cis-DDP), the most effective chemotherapeutic agent for ovarian cancer, to kill the HEY ovarian cancer cell line. We demonstrate that both EGF and LPA decrease the ability of cis-DDP to kill HEY ovarian cancer cells as assessed by colony-forming cell activity and dye reduction. Morphological changes, DNA release, and electron microscopy suggested that LPA and EGF protect ovarian cancer cells from programmed cell death induced by cis-DDP. Because LPA is present in high levels in ascitic fluid from ovarian cancer patients, and the EGF receptor is expressed by tumor cells from a significant portion of patients where it correlates with prognosis, growth factor modulation of cis-DDP-induced apoptosis may play a role in the poor prognosis associated with ovarian cancer.

Original languageEnglish (US)
Pages (from-to)1307-1313
Number of pages7
JournalClinical Cancer Research
Volume2
Issue number8
StatePublished - Aug 1 1996
Externally publishedYes

Fingerprint

Ovarian Neoplasms
Cisplatin
Intercellular Signaling Peptides and Proteins
Cell Death
Lipids
Peptides
Epidermal Growth Factor
Apoptosis
Ascitic Fluid
Cell Lineage
Epidermal Growth Factor Receptor
Electron Microscopy
Coloring Agents
Drug Therapy
Cell Line
lysophosphatidic acid
DNA
Pharmaceutical Preparations
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Peptide and lipid growth factors decrease cis-diamminedichloroplatinum- induced cell death in human ovarian cancer cells. / Frankel, Andrea; Mills, Gordon.

In: Clinical Cancer Research, Vol. 2, No. 8, 01.08.1996, p. 1307-1313.

Research output: Contribution to journalArticle

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